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Meyers-Wallen VN

Objective: To produce canine gene probes and define the onset of normal testis induction and differentiation by gene expression. These studies will provide molecular diagnostic tools for inherited reproductive abnormalities, such as hermaphroditism.

Design: Embryos were sexed by polymerase chain reaction (PCR) for Sry in genomic DNA. One urogenital ridge (UGR) per embryo (d27-37 gestation) was assayed by quantitative reverse transcription polymerase chain reaction (qRTPCR), and the other by whole mount in situ hybridization (WMISH).

Materials and Methods: Products from triplicate sample and duplicate control cDNA (RT+, RT-) templates (TaqMan Gold RT-PCR, Perkin Elmer) were quantified by automated fluorescence detection (ABI Prism 7700). Starting template was calculated from standard curves and means were compared by Student�s t test (P<.05). For WMISH, tissues were hybridized with digoxigenin-labeled riboprobes, then stained and photographed.

Results: Sry expression peaked in male UGR at d32-33. Sox9 was expressed in male but not female gonads (d32-37).MIS was expressed only in male gonads (> d32).

Conclusions: Testis induction begins with high level Sry and Sox9 expression (d31-32) and differentiation begins with MIS expression (d32).

Supported by NIH, Marilyn Simpson Trust and Dorothy R. Donnelley Faculty Development Award to the J.A. Baker Institute for Animal Health.

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Alternative Medicine
Animal Welfare
Clinical Pathology
Dental Congress 1:1
Dental Congress 1:2
Dental Congress 1:3
Dental Congress 2:1
Dental Congress 2:2
Dental Congress 2:3
Emergency & Critical Care
Feline Endocrine & Infectious Dz
Feline Gastroenterology
Feline Urology/Nephrology
Infectious & Zoonotic Diseases
Respiratory Medicine
Surgery & Sports Medicine
Oral Presentations
Morbidity Factors Of Anxiety Disorders
PTT Excision Of Ear Canal Polyps
Rabies Antibody Tests: IHA vs Elisa
Hip Luxation: Surgical Repair
Surgical Repair Of Mitral Regurg.
Dental Pulp/Dentin Deposition
Ehrlichiosis Study, Azarbaijans
B. Thuring. Effects On Trichostrongylus
Growth Plate Closure Times, Kangal
Revolution, Dog/Cat Flea Control
Electrical Stim. For Nerve Injuries
BAPN-F For Gastroc. Tendon Healing
Patch Test, Dogs; Human Allergy Stats.
Abd. Adhesion Prevention, Omenectomy
Leptospirosis Study, Tehran
Ehrlichiosis, Protein Alterations
Xenograft For Femural Fx. Repair
Omentum For Tendon Healing
Pet Behavior During Bombing
Evaluation Of Silk Suture
You are hereCanine Testis Gene Expression
Radiologic Signs, Spirocerca
Feline Interferon In FeLV/FIV Cats
HbA1c In Diabetic Dogs
Bovine Xenograft For Tibial Repair
Tibial Segmental Defect, Treatment
Bovine Bone Pin For Fracture Repair
Rabies Immunity, Tehran, Dogs
Oral Antibiotics, Owner Compliance
Excision Arthroplasty, Dogs/Cats
Ovine Xenograft For Radial Fractures
Endochondral Ossification
Intertrochanteric Osteotomy Fixation
Immunoglobuline, Atopic Dermatitis
Subclinical Dermatophytosis
Probiotic Strains
Efficacy of Revolution (selamectin)
Bordetella Bronchiseptica
Bone Cement and Graft
Femoral Head
Bacterial Infections, Urinary
Mammary Neoplasias
Bilateral Anterior Hemimelia
Adenocarcinoma, Apocrine Glands
Bone Marrow Toxicity
Carmustine, Lymphoma
Desflurane Anesthesia
Nutrition, Hyperlipidaemia
Perianal Fistula
L-Carnitine Supplementation
Poster Presentations