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Category: Dogs

Testing of Canine Fecal Samples for the Presence of Parvovirus using Real-time PCR, and Genetic Characterization of the Isolates (Study Closed)
July 16, 2008 (published)
Melissa Kennedy
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Study Start Date: 08/01/2008
Study End Date: 12/31/2008

Testing of canine fecal samples for the presence of parvovirus using real-time PCR, and genetic characterization of the isolates

The Clinical Virology Laboratory at UTCVM is investigating whether current ELISA's detect parvoviruses that may vary antigenically from the prototype CPV-2b, the most common isolate currently circulating in the USA. For this study, we are accepting fecal samples from suspect parvovirus cases (either ELISA negative or ELISA positive samples are acceptable). We will test the sample by real-time PCR, which can detect very low levels of virus in the feces; in addition we will test the sample using IDEXX SNAP CPV ELISA. If positive by the PCR assay, we will then genetically characterize the virus to determine the strain type. If negative by PCR, we will perform electronmicroscopy in order to determine if virus(es) (including heterologous viruses) is/are present.

Study Design:
Prospective, Survey

Sample Size:
Opportunistic

Inclusion Criteria:
Dogs with clinical signs compatible with canine parvoviral enteritis.

Exclusion Criteria:
Dogs with diseases other than enteritis compatible with CPV.

Study Controls:
Not applicable

Study Endpoints:
Not applicable

Samples:
Small amount of fecal sample from animals in acute stage of enteritis; store at 4oC until shipping (refrigeration); place sample in small plastic tube, bottle, or bag; double bag prior to shipping, ship on cold pack.

Files for Download:
Download the Sample Shipping form. This form is interactive and allows you to fill in the information on your computer prior to printing.

Costs/Reimbursements:
Samples are done at no charge (real-time PCR, isolate identification, electronmicroscopy).
Shipping costs are covered by the investigators – please contact investigators for specific information prior to shipping: 865-974-5643

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Full Disclosure information:

  • The study is funded by a grant from IDEXX Laboratories.
  • The investigator has declared that one of the investigators has a financial interest in a company involved in the field – Melissa Beall is an IDEXX employee.
  • The study will be published if results are negative.
  • The study will NOT be reported on VIN.
  • The authors will acknowledge VIN if the study is published.


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