Study Start Date: 07/01/2007
Study End Date: 10/31/2008

Investigation of Antigenic Causes of Vaccine-Associated Allergic Reactions in Dogs

Allergic reactions are the most common adverse event associated with vaccination in dogs. Recent research has demonstrated that small dogs in general, and several specific breeds, are at higher risk for these reactions. Laboratory studies have shown that normal dogs make antibodies against many vaccine components, even though they may not show an allergic reaction immediately following vaccination. Although the specific cause of allergic reactions in dogs remains unknown, vaccine components residual from the manufacturing process have been incriminated. Using serum from dogs of high-risk breeds, this study will compare antibody concentrations in dogs demonstrating allergic reactions versus antibody concentrations in dogs of the same breed that don't have reactions. From this study, vaccine components that stimulate allergic reactions will be identified, prompting vaccine manufacturers and/or the USDA (as regulating agency) to remove/reduce these substances and improve the safety of canine vaccines.

Study Design:
Prospective cohort

Sample Size:
250 (125 cases and 125 controls)

Inclusion Criteria:
Dachshunds, Pugs, Boston terriers, Chihuahuas, and Miniature Pinschers that have been vaccinated in the last 3 weeks and experienced an allergic (type I hypersensitivity) reaction immediately after vaccination.

Exclusion Criteria:
Other breeds; non-IgE associated vaccine reactions; vaccinated more than 21 days ago.

Study Controls:
Dachshunds, Pugs, Boston terriers, Chihuahuas, and Miniature Pinschers that have been vaccinated in the last 3 weeks but did not experience an allergic reaction after vaccination.

Study Endpoints:
Antigen-specific IgE concentrations in dogs experiencing allergic reactions will be compared with concentrations in vaccinated dogs of the same breed that didn't have reactions.

Samples:
2 ml serum desired. Mailers and testing provided free of charge.

Costs/Reimbursements:
The sample mailers, mailing, and tests are provided free with coordination.

Full Disclosure information:

• The study is funded by a grant from AKC Canine Health Foundation.
• The investigator has lectured on vaccine safety that were sponsored by vaccine manufacturers.
• The study will be published if results are negative.
• The study will be reported on VIN.
• The authors will acknowledge VIN if the study is published.