Identification of Genetic Risk in Collies and Shetland Sheepdogs with Epilepsy
Primary Investigator:
Leigh Anne Clark
Clemson University
Coinvestigators:
Lizzie Greif
Clemson University
Study Start Date: 07/01/2021
Study End Date: 07/01/2023
Identification of Genetic Risk in Collies and Shetland Sheepdogs with Epilepsy.
Epilepsy is a major health concern of many breeds, including collies, and has been a primary focus of canine genetics research; however, no genetic studies have been conducted to understand the genetic basis of epilepsy in collies and shelties. In Objective 1 we propose to conduct a genome-wide association study (GWAS) to determine if regions of the genome are associated with epilepsy in collies. As epilepsy is a genetically complex disorder, we propose a large study comprised of 50 epileptic collies and 100 healthy control collies. In Objective 2 we will generate whole genome resequencing data from epileptic and healthy collies to identify specific genetic variants that may confer risk to, or protection from, epilepsy. The long-term goal of this research is to develop a genetic test to enable selective breeding practices that will reduce the incidence of epilepsy among collies while preserving genetic variation.
Study Design:
Prospective genome analysis
Sample Size:
100 dogs of each breed
Inclusion Criteria:
- Collie dogs or Shetland Sheepdogs with a history of Seizure or Epilepsy
Exclusion Criteria:
- Dogs of other breeds
- Collies or Shelties without history of seizures
Study Controls:
Control dogs have already been identified and analyzed.
Samples:
EDTA blood sample (at least 2 cc), refrigerated and shipped to arrive Monday through Friday.
Costs/Reimbursements:
Any sample collection fees and shipping paid for by study contact.
Contact Info and Sample Shipping Info:
Lizzie Greif
Dept. of Genetics Biochemistry
Clemson University
154 Poole Agricultural Center
130 McGinty Ct.
Clemson SC 29634-0318
Email: egreif@g.clemson.edu
Phone: 864-656-4696
PI Contact Info:
Leigh Ann Clark
Dept. of Genetics Biochemistry
Clemson University
154 Poole Agricultural Center
130 McGinty Ct.
Clemson SC 29634-0318
Full Disclosure information:
The study is funded by a grant from Collie Health Foundation.
The investigator does not have a conflict of interest.
The study will be published if results are negative.
The authors will acknowledge VIN if the study is published
Date published: 07/20/2022