Assessment of Canine Distemper Using RT-PCR, Conjunctival and Enzyme-Linked Immunosorbent Assay in Turkey
WSAVA 2002 Congress
*Aykut Özkul, Aziz Arda Sancak, Ayþe Gençay, Ýbrahim Burgu
*Ankara University, Veterinary Faculty
Dýþkapý, Ankara, TR
A.Arda.Sancak@veterinary.ankara.edu.tr

OBJECTIVES

Canine distemper virus (CDV) is a member of the genus Morbillivirus in the Paramyxoviridae family, and has been known for centuries causing severe systemic disease throughout the world. In the present study, CDV was evaluated by using RT-PCR, and cytology of conjunctival scrapings, ELISA system and virus neutralization test (VNT).

MATERIALS

Nasal and ocular swabs, conjunctival scraping, and serum samples were taken from 20 canine distemper suspected stray dogs.

RESULTS

CDV RNA was detected by RT-PCR in 45% of suspected cases while dot-ELISA and virus neutralization test revealed variable results as 65% and 15%, respectively. On the other hand, crystalline inclusions were not determined in the conjunctival scraping preparations.

CONCLUSION

However, ELISA may have more advantageous in terms of estimating protective features (or titres) of the antibody response detected. we found that RT-PCR is more reliable than other methods in diagnosis of canine distemper during early stages.

Speaker Information
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Aziz Arda Sancak
Ankara University, Faculty of Veterinary Medicine
Department of Medicine
Ankara, Diskapi 06110 TR

Ýbrahim Burgu
Department of Virology, Faculty of Veterinary Medicine, University of Ankara, Turkey

Ayþe Gençay
Department of Virology, Faculty of Veterinary Medicine, University of Ankara, Turkey

Aykut Özkul
Ankara University, Veterinary Faculty
Dýþkapý, Ankara 06110 TR


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