Determination of the Required Time for Appropriate Chemical De-Epithelialization of Ileal Segment for Cystoplasty: An Animal Model
Introduction: To determine the required time for the appropriate enzymatic treatment of ileal segment to de-epithelialize its mucosa and to reduce its absorptive function for ileocystoplasty in dogs.
Materials and Methods: An experimental study was performed on 21 adult female Persian mixed breed dogs as follow. Negative control group (NCG): no ileocystoplasty was done. Positive control group (PCG): routine ileocystoplasty without enzymatic treatment of ileal segment was performed. Ileocystoplasty with 5, 10, 15, 20 or 25 minutes enzymatic treatment of ileal segment with collagenase and trypsin was performed on respective treatment group (5METG, 10METG, 15METG, 20METG and 25METG). Each group contained three dogs. The seven groups were then compared for hematologic, biochemical, and histologic changes and also glucose reabsorption by glucose absorption test.
Results: No dogs showed any signs of metabolic disturbances, biochemical and hematologic changes. Significant differences in blood glucose level (BGL) were found between groups at different times after glucose absorption test. Pair wise comparison revealed significant differences in BGL between NCG and the other groups except 25METG. No statistically significant difference was found in BGL between NCG and 25METG. The degree of histopathologic changes was correlated with duration of enzymatic treatment, so that the changes were more prominent in 25METG. No shrinkage and collagen deposition were observed.
Conclusions: Twenty-five minutes enzymatic treatment of the ileal segment is sufficient to omit the absorptive function of the augmented bladder, and is recommended for ileocystoplasty.
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