The Comparative Study of Cd4+- T Lymphocyte And Cd8+- T Lymphocyte Ratios Between Normal and Feline Immunodeficiency Virus Infected Cats
Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn University
To study and compare the lymphocyte profile of 20 normal cats with FIV infected cats.
Materials & Methods
The study of feline lymphocyte subpopulation profile was established using a whole blood lysis technique followed by flow cytometric analysis.
This study showed that cats naturally infected with feline immunodeficiency virus had altered CD4+ and CD8+ cell numbers compared to those observed in normal cats. In FIV positive cat, the % CD4+ cells decreased significantly (p<0.001). However, the CD8+ cells in FIV positive cats increased significantly (p<0.05). The CD4+/ CD8+ ratios was reduced significantly to 0.570 ± 0.341, compare with 1.610 ± 0.654 in normal cats (p<0.001). Gender did not effect the CD4+/ CD8+ ratios between normal and FIV cats. Moreover, the CD4+/ CD8+ ratios between FIV cats in asymptomatic stage and AIDS related complex were not statistically different.
Cats with FIV had lower CD4+ and CD4+/ CD8+ ratios when compared with normal cats.