Modified Choukroun’s Platelet-Rich Fibrin Used as a Topical Wound Treatment in a Captive Elongated Tortoise (Indotestudo elongata)
American Association of Zoo Veterinarians Conference 2017
Ting-Yu Chen, DVM, MSc (Wild Animal Health)
Animal Medical Center, Taipei Zoo, Taipei City, Taiwan


Few studies have been reported about the benefit of platelet-rich fibrin (PRF) in the treatment of reptiles.3,4 Most protocols require the use of specific anticoagulants and several steps to prepare PRF for use, but the preparation of Choukroun’s PRF is relatively easy.1,2 Choukroun’s PRF is derived by immediate centrifugation from collected blood without any anticoagulant added, which makes a strong fibrin matrix clot.1,2 In this study, the Choukroun’s PRF technique was modified to produce PRF for application on an elongated tortoise (Indotestudo elongata). The Choukroun’s PRF was applied once per week or every other week with a satisfactory effect. To the author’s knowledge following an electronic search of current literature, there are currently no reports of Choukroun’s PRF preparations derived from reptilian blood.3 Further studies and detailed trials are needed to evaluate the true efficacy of this protocol in reptiles. The aims of this communication are to share the clinical findings and encourage more veterinarians to institute this treatment and report their results.


The author thanks the keepers at the Conservation and Rehabilitation Center of Taipei Zoo for the daily management and care of this individual, especially Yi-Ying Chen who dedicated her time to cooperate in this treatment protocol. The author also thanks all the coworkers at the Animal Medical Center of Taipei Zoo who assisted with sample testing and treatment of this patient.

Literature Cited

1.  Choukroun J, Adda F, Schoeffler C, Vervelle A. Une opportunite´ en paro-implantologie: le PRF. Implantodontie. 2001;42:55–62.

2.  Choukroun J, Diss A, Simonpieri A, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part V: histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;101:299–303.

3.  Di Ianni F, Merli E, Burtini F, et al. Preparation and application of an innovative thrombocyte/leukocyte-enriched plasma to promote tissue repair in chelonians. PLoS ONE. 2015;10(4):e0122595.

4.  Wang HL, Avila G. Platelet rich plasma: myth or reality? Eur J Dent. 2007;1(4):192–194.


Speaker Information
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Ting-Yu Chen, DVM, MSc
Animal Medical Center
Taipei Zoo
Taipei City, Taiwan

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