An adult, captive, female shark ray (Rhina ancylostoma), which was on exhibit at the S.E.A. Aquarium, Resorts World Sentosa (RWS) in Singapore, was emaciated and pale on both the dorsal and pectoral fins. The shark ray was 230 cm long (total length), and weighed 87.6 kg. Clinical signs include dyspnea with increased frequency of the buccal pumping and ram ventilation. Blood examination indicated marked leukocytosis, anemia, and hypoxemia. Abdominal ultrasonographic findings were fluid accumulation in the abdomen, a round-shaped liver, and a distended gallbladder. Systemic bacterial infection was suspected. Combined antibiotic therapy was instituted, with ceftiofur intramuscularly, and alternate doses of marbofloxacin and amikacin intravenously. Under tonic immobility, elasmobranch-balanced salt solution was given intravenously to prevent dehydration. Daily tube feeding with 400 to 500 ml of commercially prepared feed (Mazuri® Shark/Ray meal 5D2Q) supplemented with iron for 7 days. The animal started to feed on its own after 3 days. However, anemia was still present. Oral iron supplementation was then administered daily. At 4 weeks, the animal had good appetite and was already behaving normally; and leukocytosis and anemia have been corrected.