Clinical, Laboratorial and Ultrasonographical Findings of Dogs Submitted to Bladder Augmentation Using Porcine Small Intestinal Submucosa Grafts Seeded with Cultured Homologous Smooth Muscle Cells
V.J.V. Rossetto1; L.M. Matsubara1; H.A. Miot2; P.M. Souza3; J.J.T. Ranzani1; M.J. Mamprim3; C.V. Brandão1
There are many indications for surgical repair of the bladder. Porcine intestinal submucosa (SIS) is widely used for this propose, but with some clinical limitation, as cystitis and urolithiasis. An alternative to decrease these complications is cell implantation associate to the tecidual bioengineering.
This study aimed to compare the clinical, laboratorial and ultrasonographical findings of dogs submitted to bladder augmentation using acellular and seeded SIS.
Were used 10 mixed-breed, female dogs, randomly distributed in two groups: control group (CG), constituted by five animals submitted to cystoplasty with acellular SIS; and treated group (TG), constituted by five animals submitted to cystoplasty with graft of SIS seeded with homologous smooth muscle cells. A postoperative evaluation was performed at 24 (M1) and 72 (M3) hours, and at seven (M7), 30 (M30) and 60 (M60) days.
There was no observed clinical differences between the groups. Complete blood count and biochemical exams of both groups revealed no abnormalities. The urine type I exam revealed bacteriuria only in two animals from the CG at M7 and M30. In all the cases was isolated Staphylococcus β hemolítico (p = 0,44). The ultrasonographic exam at M30 showed urinary sediment only in four animals from the CG (p = 0,05). At M60, irregularities with uroliths were visualized at the region of the SIS in only three animals from the CG (p = 0,17).
We propose that SIS associated to homologous smooth cells can decrease the potential complications inherent to acellular SIS.
Support: FAPESP, CNPq.