Evaluation of Three Test Kits for the Manual Counting of Leukocytes in Whole Blood in Wild and Captive Ring-Tailed Lemurs (Lemur catta)
American Association of Zoo Veterinarians Conference 2012

Cora L. Singleton1, DVM; Aimee Norris1, LVT; Michelle L. Sauther2, MA, PhD; Frank P. Cuozzo3, MA, PhD; Ibrahim Antho Jacky Youssouf4, PhD

1Riverbanks Zoo and Garden, Columbia, SC, USA; 2Department of Anthropology, University of Colorado, Boulder, CO, USA; 3Department of Anthropology, University of North Dakota, Grand Forks, ND, USA; 4Laboratoire de Biologie Animale et Ecologie Terrestre, Faculté des Sciences, Université de Toliara, Madagascar


The Becton Dickinson Unopette® leukocyte count test has been used as part of health evaluations of wild ring-tailed lemurs at the Beza Mahafaly Special Reserve since 2003. Production of the Unopette® was discontinued in 2009. The goal of this project was to select a Unopette® replacement test that is accurate and yields results comparable to historical Unopette® results. This project compared the Whi-pette test (Exotic Animal Solutions, Inc., Hueytown, AL 35223 USA), the LeukoChek™ test (Biomedical Polymers, Inc., Gardner, MA 01440 USA), and a 2% glacial acetic acid test with (1) an automated leukocyte count performed at a United States reference laboratory and (2) the Unopette® manual leukocyte count performed in the field.

Leukocyte count tests are considered acceptable if the results are within 15% of the standard test result.1 For the first part of this study, the standard test was the automated leukocyte count. The Whi-pette and LeukoChek™ test kits performed similarly, both with 66% of results within the acceptable range, whereas 22% of the acetic acid test results were within the acceptable range. For the second part of the study, the standard test was the Unopette® test. Compared with the Unopette® test kit, the Whi-pette and LeukoChek™ tests had 73% and 77% of results within the acceptable range respectively, whereas the acetic acid test had 69% of results within the acceptable range. The Whi-pette and LeukoCheck™ tests appear to be equally acceptable replacements for the Unopette®, whereas the acetic acid test is not an acceptable replacement.

Literature Cited

1.  Code of Federal Regulations. Title 42, Chapter IV, Subchapter G, Part 493: Laboratory Requirements. 2004.


Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Cora L. Singleton, DVM
Riverbanks Zoo and Garden
Columbia, SC, USA

MAIN : AAZV Conference : Kits for Manual Counting Leukocytes in Ring-Tailed Lemurs
Powered By VIN