Calicivirus Isolation From an outbreak of Vesicular Disease in California Pinnipeds
IAAAM Archive
E. S. Berry1; J. E. Barlough1; D. E. Skilling1; A. W. Smith1; N. A. Vedros2
1Calicivirus Research Laboratory, College of Veterinary Medicine, Oregon State University, Corvallis, OR; 2School of Public Health, University of California, Berkeley, CA

A severe outbreak of vesicular disease was observed in pinnipeds at the California Marine Mammal Center in 1984. Vesicular fluid collected from these animals was examined by direct electron microscopy and found to contain calicivirus-like particles. Samples of vesicular fluid inoculated into cell cultures (Vero cells, a primate cell line) produced cytopathic effects; passaged cell culture material was found to contain calicivirus. The isolate was cloned by 4 plaque-passages in Vero cells. Antibodies to this isolate, designated CSL-461, were found in 24/29 California sea lion pups sampled on San Miguel Island, California, in September 1984. Antibodies to CSL-461 have also been found in 2/69 bovine sera collected in Oregon in late 1984. Currently serological and biochemical studies are being performed to identify this new isolate, for comparison to previously isolated marine calicivirus serotypes.

Speaker Information
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E. S. Berry
Oregon State University
Corvallis, OR


MAIN : Session V : Calicivirus Isolation
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