Correlation of Pathology and Immune Reactivity of Splenic Tissue of Hybrid Striped Bass Infected with Mycobacterium sp.
IAAAM Archive
Stephen A. Smith; Steven D. Holladay; John L. Robertson
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA

Normal host defense mechanisms against bacterial pathogens include a variety of inflammatory, hematologic and immunologic processes. An outbreak of mycobacteriosis in a commercial hybrid striped bass culture facility allowed the correlation of disease stage with splenic immunologic parameters, including histopathology, hematopoietic cell counts and macrophage activation. Splenic macrophage metabolic oxidative burst (chemiluminescent response) and the capacity to phagocytize fluorescent microspheres were determined by flow cytometry. Cellularity of the spleen was markedly and approximately equivalently reduced in mycobacterium-infected fish relative to uninfected controls, regardless of infection stage. In contrast, splenic macrophages from infected fish displayed increased chemiluminescent responses at all stages of infection, in an approximate stage dependent manner. The phagocytic capacity of phorbol myristate acetate (PMA)-activated splenic macrophages was different from control only at the most advanced stage of infection, at which time it was significantly reduced relative to control levels.

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Stephen A. Smith, DVM, PhD
Department of Biomedical Sciences and Pathobiology
Virginia-Maryland Regional College of Veterinary Medicine
Virginia Polytechnic Institute and State University Phase II
Blacksburg, VA, USA


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