Investigating Cryopreservation Techniques for Okapi (Okapia johnstoni) Sperm
American Association of Zoo Veterinarians Conference 2008
Judilee Marrow1,2, DVM; Marcie Oliva3, LVT; Linda Penfold3, PhD
1VCA Aurora Animal Hospital, Aurora, IL, USA; VCA Berwyn Animal Hospital, Berwyn, IL, USA; 3White Oak Conservation Center, Yulee, FL, USA


Development of assisted reproductive technologies in okapi (Okapia johnstoni) could contribute to increasing genetic diversity in the captive population. The aim of this study was to evaluate the use of two common cryoprotectant agents (CPAs), glycerol (Sigma Chemical Co., St Louis, MO) and dimethyl sulfoxide (Sigma Chemical Co., St Louis, MO), at concentrations of 1%, 2%, and 4%, to determine the optimal freezing conditions for okapi semen. Samples were assessed for motility and progressive status at the following stages: at initial collection, addition of extender, cooling to 4°C, and post thaw. Prior to freezing both cryoprotectants appeared to have similar motility and forward progressive status ranging from 15–35%, average 26% with a forward status of 3.0/5. When comparing post-freezing samples the 4% glycerol dilution demonstrated the greatest post-thaw motility of 18% and forward progressive status of 3.4/5, when compared to 3–14% motility and 1.6–2.5/5 progressive forward motility in other samples. To the author’s knowledge this is the first report of cryopreservation of okapi semen.


The authors would like to thank Dr. Scott Citino and the White Oak Conservation Center staff for their assistance in collecting these samples.


Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Judilee Marrow, DVM
VCA Aurora Animal Hospital
Aurora, IL, USA

VCA Berwyn Animal Hospitals
Berwyn, IL, USA

MAIN : AAZV Conference : Cryopreservation Techniques for Okapi Sperm
Powered By VIN