Se-Il Park; Jae-Ho Jeong;Young-Mi Moon; San-Ok Kim; Jang-Yoon Choi; Kwang-Ho Jang*
The ADSCs, have the capacity for renewal and the potential to differentiate into multiple lineages of mesenchymal tissues. These cells are capable of forming bone when implanted in an appropriate scaffold.
We evaluate the effect of reconstructing bone defect by using degradable porous scaffolds seeded with ADSCs and compare the suitability of different biomaterial porous scaffolds; Hydroxyapatite/beta-Tricalcium Phosphate, Beta-Tricalcium Phosphate, Calcium Meta phosphate , Collagen-coated CMP.
ADSCs were obtained 24 adult Beagles undergoing lipectomy. The cells were isolated, cultured, labeled and seeded in vitro. The experimental group received ADSCs scaffolds and the control group received the acellular scaffolds into femoral defects, respectively. The bone blocks were retrieved at 4, 8, 12 weeks after the surgery for radiographic, biochemistry, histomorphologic analysis.
Histological analysis revealed that implants loaded with autologous ADSCs, had greater amounts of bone within the available pore space than the acellular implants, especially in Calcium Meta phosphate (CMP) group.(p < 0.05). Newly formed bone was observed in the healed defects at 12 weeks and the osteoblastic differentiation of implant with ADSCs was much more than that of the control.
The harvested ADSCs possess the ability to form new bone tissue when seeded onto CMP, which shows the potential of using this method to repair large bone defect clinically.