Canine Herpes Virus Infection: A Sero-Epidemiological Survey in Tunisia and Estimation of Vaccine Efficiency
World Small Animal Veterinary Association World Congress Proceedings, 2006
C. Ahmed1, F. van Gool2, M. Rmili3, F. Kallel1, F. Landolsi1, S. Haddad3
1Veterinary School, Sidi Thabet, Tunis, Tunisia; 2Merial Laboratory, Lyon, France; 3Centre Canin, Bizerte, Tunisia

We carried out, in the first part, a canine herpes virus infection sero-epidemiological survey by ELISA test on 49 reproductive bitches aged 2.9 ± 1.7 years and coming from Bizerte and Tunis regions. This survey has revealed a seropositive rate of 37%. No significant differences were found according to the sanitary status or the age of the dogs in the breeding units. In the second part, we carried out a medical prophylaxis against canine herpes virus infection in a canine centre. For that, we have used EURICAN® herpes virus vaccine produced by MERIAL Laboratories, Lyon, France. This study allows us to estimate vaccine efficiency by observing its impact on neonatal mortality. Twenty three reproductive females housed at a canine center were included in this study. These bitches were divided into 3 groups: The First group (control group) was made up of 6 bitches that did not receive any herpes virus vaccine but instead, they received a vaccine against other diseases (Distemper, hepatitis, leptospirosis, parvoviruses and rabies). The second group: contains 10 females that have received a single injection of herpes virus vaccine at the end of pregnancy period according to the protocol proposed by Lanting (2004). The third group: comprises 7 bitches that have received vaccination according to the following protocol (Poulet et al. 2001):

 First injection 10 days after mating

 Second injection 10 days before delivery date.

The result of vaccinations has shown a decrease in neonatal mortality rate. In fact, in the control group (Group1) a neonatal mortality rate was of 43.33%, while in the vaccinated groups it was of 23.4% and 7% respectively in the second and the third group.

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C. Ahmed
Veterinary School
Sidi Thabet
Tunis, Tunisia

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