The aim of this study is to evaluate the response attachment loss, periodontal pocket and II and III furcation lesion of tooth 3 and 6 month after application of collagen cell-binding peptide (PepGen P-15®). Twenty four dogs from the FMVZ-USP Veterinary Hospital were anesthetized in order to periodontal treatment and 91 tooth faces with attachment loss was treated. From it, 45% (41) received P-15 and 55% (50) constituted control group that received conventional treatment (flap and root planning). Eighth tooth had furcation lesions. Five received the peptide and 3 do not. The procedure was documented by radiography and photography from the periodontal probing. After 3 and 6 month, the animals were re-anesthetized in order to realize new photography, radiography and periodontal probing exams. From furcation tooth treated with P-15, two exhibit reduction of furcation degree, two do not change it condition and one had furcation enhanced after 6 month. The conventional treatment group presents 1 tooth with furcation reduction and no changes in 2 teeth. The 41 attachment loss faces that received graft material exhibit 40% of regeneration rate after 6 month. The control faces do not change it attachment level. The palatal face present the better regeneration rates (40%) and the canines and molars were the better tooth responses (57.14% and 65%, respectively). There was no post-surgical infection related to oral home care, despite the fact that just one owner (4.16%) had reported diary tooth brush on his pet. This data permits to conclude that the P-15 helps the more rapidly periodontal structures re-attachment e regeneration, including alveolar bone. Its application is easy and practical and the post-surgical complications incidence is poor. Nevertheless, more studies and researches are necessary to evaluate the formed bone and periodontal ligament amount and the quality.