Comparative Study of Parasites in Pacific Bluefin Tuna, Yellowfin Tuna and Others Scombrid Fishes from Panamá - An Identification of a New Parasite: Kudoa rayformis sp. nov.
IAAAM 2017
Lissette T. Trejos-Lasso1,3*; Sho Shirakashi2; Amado Cano1
1Aquatic Resources Authority of Panama, Panama City, Panama; 2Fisheries Laboratory, Kinki University, Shirahama, Nishimuro, Wakayama, Japan; 3Environmental Ministry-Cost and Sea Department, Panama, Panama City

Parasitic diseases have recently become significant threats to tuna aquaculture in Australia and Japan. The aim of our study is to survey parasites in wild yellowfin tuna (YFT) and other Scombrid fish to identify parasite species that can be potential risks in YFT culture in Panama. Wild YFT and Scombrids were caught by fishing or obtained from local fishermen. Each fish were measured and their organs including gills, hearts, brains, gastro-intestinal tract, muscle, kidney, and/or liver were examined under a light stereomicroscope. Any parasite specimen found were photographed and fixed in ethanol for morphological and molecular analyses. In wild YFT, we found Didymocystes in gills arch and gastrointestinal mucosa. Trichodina and Copepods were found in gills and Digeneans, Acantocephalans, Nematodes and Cestodes were obtained from stomach. In addition, we found scuticociliate possibly Uronema sp. from a brain of one YFT. No parasites were detected from the YFT juveniles reared in sea cages for about 4 weeks at Achotines Experimental Station. From wild Scombrids, we identified several parasites including gill flukes and trematodes. Furthermore, we detected myxozoan infection in the trunk muscle of Pacific sierra Scomberomorus sierra caught off the coast of Tonosi, Panama. Morphological and molecular analyses reveal this is a new species of genus Kudoa and we described this species as Kudoa rayformis n. sp. The species formed pseudocysts in myofibers and infection was subclinical. The myxospores possessed four polar capsules and spore valves, one of which had a distinct filamentous extension. This unique morphological characteristic of the myxospore validated this as a new species of Kudoa. In the study, we did not detect any species, except for scuticociliate in brain, that can be a potential problem in tuna culture in Panama. However, more studies are necessary for detailed risk assessment of parasitic diseases in cultured YFT.

Acknowledgements

The authors we thanks to Licdas. Maruslybel López, Diana Pérez, Anna Núñez, Ing. Amado Cano, Ing. Dario Sandoval, Leyson Solís. Achotines Laboratory fishing staff, Captain: Cota, Daniel Mancilla, Carlos, Agustin, Dario, Inn. El Ciruelo Beach and Arenal Beach Fishermans (Pepino, Teto, Nego). Fish Patology Laboratory. Staff, to Shirahama Fisheries Experimental Station, Kinki University 2015. (Dr. Shin Sam Phil, Katzuki, Hamano, Nishimura) in special to Sho Shirakashi, PhD and Keitaro Kato, PhD, for the photografy. Sabbah University, Malassia students (Aisha Faisal).

* Presenting author

Literature Cited

1.  Guía sobre los principales Parásitos presentes en productos pesqueros: Técnicas de Estudio e Identificación, Ministerio de Agricultura, Alimentación y Medio Ambiente. Secretaría General Técnica Centro de Publicaciones Catálogo de Publicaciones de la Administración General del Estado: http://publicacionesoficiales.boe.es, Madrid, España, 2012;104.

Journal Article

2.  Flores-Crespo J, Flores CR. 1993. Principales Trematodos y Cestodos de importancia económica en acuicultura. En: Tópico de parasitología animal cestodos y trematodos. Universidad Nacional Autónoma del Estudio de Morelos. II:13–36.

3.  Moran JDW, Whitaker DJ, Kent ML. 1999. A review of the myxosporean genus Kudoa meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries. Aquaculture. 172:163–196.

4.  Bueno P, Juana M, Ferre Pérez, Ignacio. 1997. Parásitos del Pescado. Universidad de León Departamento de Patología Animal: Sanidad Animal. Junta de Castilla Y León Consejería de Sanidad y Bienestar Social.

5.  Rodríguez Llanos J. 2012. Parásitos del atún rojo del Atlántico (Thunnus thynnus) en granjas de engorde del Mediterráneo español. Patologías y uso como biomarcadores. Tesis de Máster Interuniversitario de Acuicultura, Universidat Politécnica de Valencia.

6.  Ruiz de Ybañez, R, Peñalver, J, Martínez-Carrasco C, del Río L, Dolores EM, Berriatua E, y Muñoz P. 2011. Blood fluke infection of cage reared Atlantic bluefin tuna Thunnus thynnus in West Mediterranean. Fish Pathology. 46(3):87–90.

7.  Sawada Y, Okada T, Miyashita S, Murata O, y Kumai H. 2005. Completion of the Pacific Bluefin tuna Thunnus orientalis (Temminck et Schlegel) life cycle. Aquaculture Research. 36;413–421.

8.  Sang Phil Shin, Nguyen VG, Jeong JM, Jun JW, Kim JH, Han JE, Baeck GW, Park SC. 2014. The phylogenetic study on Thelohanellus species (Myxosporea) in relation to host specificity and infection site tropism. Molecular Phylogenetics and Evolution. 72:31–34.

9.  Sang Phil Shin, Sho Shirakashi, Shogo Hamano, Keitaro Kato, Lissette Trejos Lasso, Hiroshi Yokoyama. 2016. Phylogenetic study of the genus Kudoa (Myxozoa: Multivalvulida) with a description of Kudoa rayformis sp. nov. from the trunk muscle of Pacific sierra Scomberomorus sierra. Molecular Phylogenetics and Evolution. 98;337–345.

10. Shirakashi S, Kishimoto Y, Kinami R, Katano H, Ishimaru K, Murata O, Itoh N, y Ogawa K. 2012. Morphology and distribution of blood fluke eggs and associated pathology in the gills of cultured Pacific bluefin tuna, Thunnus orientalis. Parasitology International. 61;242–249.

11. Yokoyama H. 2003. A review: Gaps in our knowledge on myxozoan parasites of fishes. Fish Pathology. 38:125–136.

12. Yokoyama H, Itoh N. 2005. Two multivalvulid myxozoans causing postmortem myoliquefaction: Kudoa megacapsula n. sp. from red barracuda (Sphyraena pinguis) and Kudoa thyrsites from splendid alfonso (Beryx splendens). Journal of Parasitology. 91:1132–1137.

13. Yokoyama H, Suzuki J, Shirakashi S. 2014. Kudoa hexapunctata n. sp. (Myxozoa: Multivalvulida) from the somatic muscle of Pacific bluefin tuna Thunnus orientalis and re-description of K. neothunni in yellowfin tuna T. albacares. Parasitology International. 63:571–579.

  

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Lissette T. Trejos-Lasso
Aquatic Resources Authority of Panama
Panama City, Panama


MAIN : Special Spanish Session: Aquatic Animal Medicine : Comparative Study of Parasites in Tuna
Powered By VIN
SAID=27