In vitro Viability of Canine Oocytes
World Small Animal Veterinary Association World Congress Proceedings, 2003
M.De los Reyes1, L. Tello2, P. Miranda1
1Animal Reproduction Laboratory; 2Small Clinical Teaching. Faculty of Veterinary Sciences. University of Chile, Santiago, Chile

Objectives

The viability of oocytes should be a important selection parameter for successful in vitro culture systems. This study evaluated the viability of canine oocytes before and during in vitro culture period.

Material & Methods

Bitch oocytes were collected from ovariohysterectomy; the ovaries were sliced and released oocytes were washed with PBS The oocytes were placed in 50µl drops of maturation media: (TCM 199 with 10% fetal calf serum, 20 mM Hepes and 10 UI/ mL of hCG) for 72 h at 39°C with 5% CO2 in air. Oocyte viability was assessed by fluorescein diacetate staining (FDA) under epifluorescence microscope before cultured and every 24 h during culture period. Green fluorescence indicated cell viability.

Results

Before culture 399/450 (88,6%) showed fluorescence; during culture period the cell viability was: 62/67 (92.5%); 63/73 (86.3%) and 80 /113 (70.7%), at 24, 48 and 72 h respectively.

Conclusion

A high percentage of cell viability can be obtained from canine ovaries which are maintained during culture in vitro.

Supported by Grant 1030380 FONDECYT.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

M. De los Reyes
Animal Reproduction Laboratory
University of Chile
Santiago, Chile


MAIN : Reproduction : Viability of Oocytes
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