Development of the Polymerase Chain Reaction (PCR) for the Detection and the Prevalent Study of Psittacine Beak and Feather Disease Syndrome Virus (PBFDV) Infection in Cockatoo
World Small Animal Veterinary Association World Congress Proceedings, 2003
Supranee Boonnontae1; Theerapol Sirinarumitr2,3; Worawidh Wajjawalku2,1
Veterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University; 2Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University; 3Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus
Bangkok, Thailand

Objectives

To develop the polymerase chain reaction for the detection of PBFDV and to study the prevalence of PBFD in Thailand

Materials & Methods

Skin biopsy from a Sulphur Crested Cockatoo that positive for PBFDV inclusion bodies was subjected to DNA extraction and the polymerase chain reaction (PCR). The PCR product was sequenced and compared with the sequence published on GenBank. During 2001-2002, feather follicles from 34 suspected cases of PBFDV infection were used to perform PCR.

Results

PCR product from the skin biopsy from a Sulphur Crested Cockatoo had approximately 900 bp in size. The sequence comparison result of this PCR and the sequence published on GenBank was similar. Nineteen out of thirty four suspected cases of PBFDV infection were positive.

Conclusion

This result showed that PBFDV infection in Cockatoo was common in Thailand.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Theerapol Sirinarumitr
Department of Pathology
Faculty of Veterinary Medicine
Kasetsart University


Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.

CONTACT US

777 W. Covell Blvd., Davis, CA 95616

vingram@vin.com

PHONE

  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357
SAID=27