Detection and Differentiation of CPV Type2 from CPV Type2a/2B By Multiplex PCR
World Small Animal Veterinary Association World Congress Proceedings, 2003
K. Siripattarapravat1; A. Klinkajorn1; T. Sirinarumitr1,2
1Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok; 2 Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen, NakornPathom
Thailand

Objectives

 To develop multiplex PCR for identification of CPV and differentiation of CPV 2 from CPV 2a/2b.

 To develop a rapid, sensitivity and specificity tool for routinely diagnosis of CPV infected dogs.

Materials & Methods

Samples from various commercial CPV vaccine strains, both CPV2 and CPV2a/2b strain, and diarrheic dog's feces, clinically suspected as CPV infection, were performed DNA extraction. These extracts were then used as DNA templates. Two set of primers, CPV-NS1 (common primers for CPV) and CPV2a/2b-VP2 (strain specific primers), were independently tested for each PCR and, consequently, adjusted a condition for multiplex PCR. The results were visualized by agarose gel electrophoresis and UV illumination.

Results

NS1 primers gave the positive results for all vaccine strains and 10 of 12 fecal samples. However, VP2 primers gave the positive results for the vaccine using CPV2a or CPV2b and 10 positive fecal samples. Multiplex PCR using NS1 and VP2 primers also gave the similar results.

Conclusion

The multiplex PCR could be used to detect and differentiate CPV2 from CPV2a/2b from the feces of vaccinated and infected dogs.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

K. Siripattarapravat
Department of Pathology, Faculty of Veterinary Medicine
Kasetsart University
Bangkok Thailand


MAIN : Oral Presentations : CPV Type2 vs CPV Type2a/2B
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.

CONTACT US

777 W. Covell Blvd., Davis, CA 95616

vingram@vin.com

PHONE

  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357
SAID=27