The incidence of fracture in cat, especially stray cats, is quite high in Indonesia. Among the type of fractures, the comminuted fracture is still considered challenging to handle. In this case, the demineralized freeze-dried bone allograft (DFDBA) can be an option to replace the missing part of the bone.
This research is aimed to evaluate cell viability of DFDBA by in vitro study using MTT assay.
The demineralized freeze-dried bone allograft (DFDBA) was produced from feline (cadaver) bones. The long bones of extremities were used as raw materials. Both cancellous and cortical bones were collected and processed into 3 different sizes of 20, 40, and 60 mesh for each type of the bone. Sterilization of DFDBA was conducted using gamma radiation with doses of 15 kGy and 25 kGy to each type and size material produced resulting in 12 DFDBA variants. The bone graft produced was then evaluated with the MTT assay using CPAE cells in D-MEM.
The results show that the highest cell viability (80.2%) lead by DFDBA derived from cortical bones with 20 mesh in size and 15 kGy radiation dose. Followed by 20 mesh DFDBA derived from cortical bone sterilized by 25 kGy with 72.8% cell viability.
From these results, we concluded that the CPAE cell growth was affected by the type of the bone, particle size, and radiation dose. The highest cell viability (lowest cell growth inhibition) was found in 20 mesh DFDBA derived from the cortical bone sterilized by 15 kGy gamma radiation.