The Treatment of Platelet-Rich Plasma Injections for Dogs with Osteoarthritis and the Results of MMP-2,9 and Cytokine Profiles
Plasma rich platelet may have a favorable effect on OA.
The study was to determine the levels of MMP-2 and 9, IL-1α, IL-6, IL-10, TNF-α and PGE2 in the synovial fluids after intra-articular injection of an autologous PRP for the treatment of osteoarthritis in dogs.
Twenty dogs were used as a materials. Fourteen dogs were used as osteoarthritis groups and 6 dogs were used as a control. The dogs were randomly assigned to a treatment or control group. Double centrifuged method will be used to obtain platelet-rich plasma. Lameness, pain assessment and arthrocentesis were done at days 0, 1, 3, 5, 7, 15 and weeks 4, 8 and 12.
MMP-2 and 9 enzyme bands were inhibited after PRP treatment group in 30 days following injection and there were no change in saline (0.9% NaCl) injected group. The TNF-α had observed a slight decline during the first week. In the present study the pro-inflammatory IL-6 levels remained higher in the treatment group. IL-1β levels was slight decreased while TNF-α levels was decreased in 5th days after PRP injection. In IL-6 and IL-10 levels increased with 60 and 90 days.
PRP applications inhibited MMPs activity and some inflammatory parameters in synovial fluids. This can reduce the signs and symptoms of inflammatory conditions. Cytokines levels are decreased within a week after intra articular injection. Therefore, PRP should be injected several times at regular intervals instead of a single application. PRP is an effective and safe method for treatment of OA.
This work was supported by TUBITAK (Proj. No. 2130175).