With the advent of cell therapy, the search for this type of therapy has grown exponentially; however, specialized laboratories are not always found near the clinics, thus having the need to transport these cells without incurring viability loss. Keeping in mind the current needs for commercialization of this cell type, the study aims to conduct a cell viability comparative between different conditioning methods and times after the preparation of mesenchymal stromal cells, for use in cellular therapy. Ten samples of mesenchymal stromal cells were used for quantification of total cells, viable and nonviable cells, and the samples conditioned in 72 sterile syringes containing 0.30 ml of PBS 1x plus cells. The syringes were randomly divided into three environments: refrigerator, Styrofoam box with ice, Styrofoam box without ice, and at predetermined times viability tests were performed using the trypan blue method. It was observed that in the environments "refrigerator" and "Styrofoam box with ice," cell viability remained higher than 90% at all times with the exception of moment T48, already in the environment "Styrofoam box" viability remained below 90% in moments T8, T10, T12, T24 and T48. It was found that temperatures from 2°C to 8°C are considered best for conditioning the cells during transportation, maintaining good cell viability for 24 hours.