Genomic Analysis of the CMAH Gene in a Blood Type B Cat
World Small Animal Veterinary Association World Congress Proceedings, 2015
O. Toshinori1; H. Taniguchi1; S. Ezoe1; K. Ochiai1; T. Sako2; D. Azakami2; M. Bonkobara3; S. Tsuchida4; C. Udagawa1
1Department of Basic Science, Nippon Veterinary and Life Science University, Tokyo, Japan; 2Department of Veterinary Nursing, Nippon Veterinary and Life Science University, Tokyo, Japan; 3Department of Veterinary Clinical Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan; 4Department of Comprehensive Education in Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan

Introduction

Knowledge of the cat AB blood group system is important in specifically cat transfusion studies, as antigen mismatching can cause acute hemolytic transfusion reactions. Such reactions occur when naturally occurring alloantibodies in the serum react with the antigens of the cat blood types A, B, and AB which are expressed on the surfaces of red blood cells.

Objectives

Mutations of CMAH gene have been reported in the blood type B cats. In our previous study, we found a blood type B cat with genotype revealed in blood type A. Then, we show here the detailed genomic structure of CMAH gene in this case

Methods

The nucleotide sequences of the coding region and 5'UTR of the CMAH gene were determined direct sequence analysis from genomic DNA in blood type B cat with genotype of AA. Blood types were determined using RapidVet-H Feline Blood Type cards.

Results

We redetermined the CMAH genotypes of 6 SNPs (5'UTR nt-217, 5'UTR nt-371, Ex2 nt139, Ex3 nt265, Ex4 nt324, and Ex13 nt1600) and 1 indel (5'UTR nt-53) in a blood type B cat, which showed the homozygous haplotype revealed in blood type A. In addition, we found the missense mutations in the coding region of the CMAH gene. The haplotype was suggested to heterozygous.

Conclusions

The determination of the blood type by DNA testing needs further study for genetic association between CMAH variants and phenotypes in cats.

  

Speaker Information
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O. Toshinori
Department of Basic Science
Nippon Veterinary and Life Science University
Tokyo, Japan


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