Rapid Immunocytochemistry for the Detection of Cytokeratin and Vimentin: Assessment of Diagnostic Value in Neoplastic Diseases of Dogs and Cats
World Small Animal Veterinary Association World Congress Proceedings, 2015
A. Yabuki; M. Inoue; M. Sawa; A. Setoguchi; S. Tokunaga; K. Kushida; M. Kohyama; O. Yamato
Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan

Introduction

Immunocytochemistry (ICC) is an advanced diagnostic technique used in the field of veterinary cytology. We recently developed a rapid-ICC technique for the detection of cytokeratin and vimentin, which helps to determine whether tumor cells were of epithelial or mesenchymal origin. However, the diagnostic value of this rapid-ICC in neoplastic diseases of dogs and cats is yet to be assessed.

Objective

This study aimed to assess the diagnostic accuracy of the rapid-ICC technique in comparison with standard immunohistochemistry (IHC).

Methods

Air-dried smear samples and formalin-fixed paraffin (FFP) sections were prepared from tumors excised from dogs and cats. Smear samples that were used for rapid ICC were stored at -30°C until use (for a few days to 56 months). The cytokeratin and vimentin signals produced by the smear samples that were processed using the rapid ICC technique were compared with those from FFP sections processed using the standard IHC technique.

Results

All smear samples (n = 34) that were stored for up to 46 months showed clear immunopositive signals for cytokeratin and vimentin with rapid-ICC; however, two samples that were stored for 56 months emitted low signals. In samples that were stored up to 24 months (n = 32), the correlation between rapid-ICC and standard-IHC was 81.3% (13/16) in epithelial tumors, 100% (7/7) in mesenchymal tumors, and 100% (9/9) in round-cell tumors.

Conclusion

The rapid-ICC technique can be used as a diagnostic and research tool for neoplastic diseases in dogs and cats.

  

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

A. Yabuki
Joint Faculty of Veterinary Medicine
Kagoshima University
Kagoshima, Japan


MAIN : Oncology : Detection of Cytokeratin & Vimentin
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.

CONTACT US

777 W. Covell Blvd., Davis, CA 95616

vingram@vin.com

PHONE

  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357
SAID=27