Galectin-3 as a Fibrosis Marker in Dogs with Degenerative Mitral Valve Disease
World Small Animal Veterinary Association World Congress Proceedings, 2015
S. Sakarin1; S. Surachetpong1; A. Rungsipipat2
1Veterinary Medicine, Chulalongkorn University, Bangkok, Thailand; 2Veterinary Pathology, Chulalongkorn University, Bangkok, Thailand

Introduction

Cardiac fibrosis is a pathological change in human and canine cardiovascular disease. Galectin-3 (Gal-3), a marker of cardiac fibrosis, is upregulated in heart disease patients. A use of Gal-3 to detect cardiac fibrosis in degenerative mitral valve disease (DMVD) dogs has not been studied.

Objectives

The aims of this study were to determine the expression of Gal-3 in cardiac muscles and measure level of plasma Gal-3 in CHF dogs with DMVD.

Methods

Control (n = 10) and DMVD (n = 12) cardiac samples from necropsy dogs with age > 6 years and weight < 15 kg were stained with Masson trichrome and Gal-3 immunohistochemistry. Gal-3 concentration was measured in plasma samples from control (n = 19) and DMVD (n = 27) dogs by ELISA test kits.

Results

Fibrosis area was higher in DMVD than normal dogs (p < 0.01), apparently in subendocardium and papillary muscles (Figure 1). Gal-3 was overexpressed in DMVD than normal dogs (p < 0.01), particularly in subendocardium (Figure 2). Plasma Gal-3 concentration was significantly increased in DMVD (1.498; 0.874–2.360 ng/ml) compared to normal dogs (0.420; 0.271–0.630 ng/ml) (p < 0.01).

Figure 1. Subendocardial fibrosis in normal (A) and DMVD dogs (B)

Masson trichrome stain, 20x magnification
 

Figure 2. Gal-3 expression in papillary (A, B) and LV (C, D) of normal and DMVD dogs, respectively

LSAB, IHC, Mayer's hematoxylin, 20x magnification
 

Conclusions

Gal-3 expression is associated with cardiac fibrosis. Gal-3 could be a potential candidate marker of cardiac fibrosis in DMVD dogs.

  

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

S. Sakarin
Veterinary Medicine
Chulalongkorn University
Bangkok, Thailand


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