Study of the Thermostability of Rabies Virus ERA/SAD on Different Stabilizers Medium
World Small Animal Veterinary Association World Congress Proceedings, 2014
F. Belakehal1; M. Brahimi2; A. Hallab2; T.M. Hamdi1
1Laboratory of Food Hygiene and Quality Assurance System, École Nationale Supérieure Vétérinaire, Algiers, Algeria; 2Service de la rage, Institut Pasteur d' Algérie, Algiers, Algeria

The thermostability of rabies virus was studied by subjecting the ERA strain produced in Vero cells to accelerated aging (7 days at 37°C) in the presence of stabilizers.

The analysis of the obtained results by in vivo titration method (inoculation of rabies virus to young mice) revealed that the rabies virus ERA/Vero keeps well without stabilizers at -20°C or -70°C, fairly well to +4°C, but it is sensitive to temperatures above 25°C and 37°C. The rabies virus ERA/Vero stored at 37°C for 7 days is found with an acceptable title. Apart from the PBS and the saccharose at 2% carried by the Enders buffer, all the other media used in this study: Enders buffer, sorbitol at 1% carried by Enders buffer, sorbitol at 4% carried by the PBS, sorbitol at 4% carried by distilled water, have a stabilizing effect on the rabies virus ERA/Vero after 7 days of incubation at 37°C. The best stabilizing effect on the rabies virus is obtained with the sorbitol at 4% carried by the PBS. The saccharose was ineffective; its use to a concentration at 2% carried by the Enders buffer gave a negative effect on rabies virus. The sorbitol solution at 4% carried by the PBS can be an ideal formulation, adequate and economical to be incorporated in vaccine preparations, in order to reach the production of a rabies thermostable vaccine.

  

Speaker Information
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T.M. Hamdi
Laboratory of Food Hygiene and Quality Assurance System
École Nationale Supérieure Vétérinaire
Algiers, Algeria


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