Acellular SIS and Seeded with Homologous Smooth Muscle Cells for Bladder Augmentation in Dogs: Histological Comparative Study
World Small Animal Veterinary Association World Congress Proceedings, 2014
V.J.V. Rossetto1; F. Grandi2; N.S. Rocha2; H.A. Miot3; J.J.T. Ranzani1; L.S.L.S. Mota4; C.V.S. Brandão1
1Departament of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, Brazil; 2Departament of Clinical Veterinary Medicine, School of Veterinary Medicine and Animal Science, UNESP, Botucatu, Brazil; 3Departament of Dermatology, School of Medicine, UNESP, Botucatu, Brazil; 4Departament of Genetics, Biosciences Institute, UNESP, Botucatu, Brazil

Introduction

Bladder reconstruction using gastrointestinal segments may result in infection, urolithiasis and retraction of the graft. An alternative to decrease these complications and promote more accurately repair is the cell implantation.

Objectives

The aim of the present study was to compare by histological analysis the anatomic repair of bladders augmented using acellular and seeded SIS.

Methods

Were used 10 mixed-breed, female dogs, distributed in two groups: control group (CG), constituted by five animals submitted to cystoplasty with acellular SIS; and treated group (TG), constituted by five animals submitted to cystoplasty with graft of SIS seeded with homologous smooth muscle cells. The cells were obtained from bladder fragments during the cystoplasty of CG animals. Immunocytochemistry technique was performed to confirm the cellular type. After 60 days postoperatively, all dogs were submitted to a biopsy of the bladder wall, destined two distinct regions: a) central region of the cystoplasty; and b) transition region between the neoformed tissue and original adjacent bladder. Anatomic repair was by histological staining for H/E and Masson's trichrome, processed by the ImageJ software using the k-means clustering plugin.

Results

On Table 1 is presented the average percentage of the muscle and collagenous tissues of both groups.

Conclusions

We propose that SIS associated to homologous smooth cells can improve the quality of bladder tissue repair.

Support: FAPESP, CNPq

Table 1. Amount of muscle and collagenous tissue in the central and transitional (trans.) samples of animals from the control (CG) and treated (TG) groups

  

Control

Treated

  

Region

N

Average

N

Average

P value

Muscle-central

5/5

0,30 ± 0,20

5/5

0,56 ± 0,07

0,04

Muscle-trans.

5/5

0,54 ± 0,04

5/5

0,68 ± 0,07

0,07

Collageno-central

5/5

0,70 ± 0,20

5/5

0,44 ± 0,07

0,04

Collageno-trans.

5/5

0,46 ± 0,04

5/5

0,32 ± 0,07

0,07

  

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

C.V.S. Brandão
Department of Veterinary Surgery and Anesthesiology
School of Veterinary Medicine and Animal Science, UNESP
Botucatu, Brazil


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