Detection, Diversity and Epidemiology of Marine Mammal Astroviruses
IAAAM 2009
Rebecca Rivera1; James F.X. Wellehan2; Stephanie Venn-Watson3; Eric Jensen4; Kevin P. Carlin4; Frances M.D. Gulland5; Randall S. Wells6; Judy St. Leger7; Megan Stolen8; Wendy NokeDurden8; Hendrik Nollens1,2
1Hubbs-SeaWorld Research Institute, San Diego, CA, USA; 2Marine Mammal Health Program, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA; 3Navy Marine Mammal Program Foundation, San Diego, CA, USA; 4U.S. Navy Marine Mammal Program, San Diego, CA, USA; 5The Marine Mammal Center, Fort Cronkhite, Sausalito, CA, USA; 6Chicago Zoological Society c/o Mote Marine Laboratory, Sarasota, FL, USA; 7SeaWorld, San Diego, San Diego, CA, USA; 8Hubbs-SeaWorld Research Institute, Orlando, FL, USA

Abstract

Astroviruses are enteric RNA viruses belonging to the family Astroviridae. Electron microscopic analysis of routine fecal samples from one bottlenose dolphin (Tursiops truncatus), two captive managed and one free-ranging California sea lions (Zalophus californianus), and one Steller sea lion (Eumetopias jubatus) showed particles consistent with Astroviruses. Degenerate primers were designed targeting conserved regions of astroviral genomes. Phylogenetic analysis of the resulting sequences revealed five distinct astrovirus species, significantly expanding known astroviral diversity.

A serosurvey of managed-collection and free-ranging dolphins using a peptide-based ELISA demonstrated that dolphins greater than 25 years old had higher antibody levels compared to dolphins younger than 5, and samples collected during April through June had higher antibody levels compared to the other three quarters (P = 0.01). Dolphin astrovirus (TtastV-1) appears to be a common virus that follows epidemiology similar to astroviruses in other species. Serologic evidence of exposureto dolphin astrovirus was found in both managed-collection and free-ranging dolphins.

In humans, astroviruses are a leading cause of viral diarrhea in young children,1 and astroviruses have been found in 10% of diarrheic cats.2 Although we were unable to attribute clinical signs to the presence of the astroviruses, it is likely that they have the potential to cause enteritis in marine mammal hosts. Their stability in seawater and the diversity found in marine mammals suggests that the marine environment may be important in astroviral ecology.

Acknowledgements

The authors would like to thank Dr. Pam Yochem as well as the staffs from all of our collaborating institutions. This work was funded by research grant No. N00014-06-1-0250 to HN from the Office of Naval Research.

References

1.  Dennehy P.H., S.M. Nelson, S. Spangenberger, J.S. Noel, S.S. Monroe, and R.I. Glass. 2001. A prospective case-control study of the role of astrovirus in acute diarrhea among hospitalized young children. J Infect Dis 184: 10-15.

2.  Marshall J.A., M.L. Kennett, S.M. Rodger, M.J. Studdert, W.L. Thompson, and I.D. Gust. 1987. Virus and virus-like particles in the faeces of cats with and without diarrhoea. Aust Vet J 64: 100-105.

 

Speaker Information
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Rebecca Rivera
Hubbs-SeaWorld Research Institute
San Diego, CA, USA


MAIN : Epidemiology : Astroviruses
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