Dexamethasone Induces Apoptosis in Proliferative Canine Tendon Cells and Chondrocytes
World Small Animal Veterinary Association World Congress Proceedings, 2008
M.A. Hossain; Bibek Chandra Sutradhar, DVM, MS; J. Park; S.H. Choi; G. Kim
Laboratory of Veterinary Surgery, College of Veterinary Medicine, Chungbuk National University
Heongduk-gu, Cheongju, Chungbuk, Korea

Dexamethasone (Dexa) has been commonly used in humans and domestic animals, particularly to treat tendon injuries and cartilage degeneration, but is often associated with tendon rupture and impaired tendon and cartilage healing. In the present study we investigated Dexa's in vitro effects on the growth of cell proliferation and the induction of apoptosis in canine Achilles tendon cells and chondrocytes. Cell proliferation after treating with Dexa for 2-6 days was quantified by a 2,3-bis{2-methoxy-4-nitro-5-sulfophenyl}-2H-tetrazolium-5-carboxyanilide inner salt assay (XTT).

The results showed that Dexa could inhibit the proliferation of tendon cells and chondrocytes at increasing concentrations (0.1-50 µg/ml) in compared with untreated cells. Cell apoptosis was induced by Dexa, as evidenced by the typical nuclear apoptosis using Hoechst 33258 staining. Dexa increased the apoptosis of canine tendon cells and chondrocytes in a time-dependent manner. In canine tendon cells and chondrocytes treated with 25 and 50 µg/ml concentration of Dexa the number of condensed apoptotic nuclei was significantly increased. In addition, culturing with Dexa and the glucocorticoid receptor blocker mifepristone significantly arrested apoptosis of tendon cells and chondrocytes.

Based on our in vitro data, we speculate that in vivo treatment with glucocorticoids may diminish the proliferation of tendon and cartilage cells by increasing apoptosis and suppressing the proliferation. Our findings suggest that Dexa could be used with caution in dogs with articular or tendon problems.

Speaker Information
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G. Kim, DVM, MS
Laboratory of Veterinary Surgery, College of Veterinary Medicine
Chungbuk National University
Cheongju, Chungbuk, Korea

Bibek Chandra Sutradhar, DVM, MS
Laboratory of Veterinary Surgery, College of Veterinary Medicine
Chungbuk National University
Cheongju, Chungbuk, Korea

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