Performance of an in-Clinic ELISA Test, Snap® Feline TripleTM, for the Detection of Antibodies to Feline Immunodeficiency Virus, Feline Leukemia Virus Antigens and Feline Heartworm Antigens in Cats
ACVIM 2008
C. Mainville; B. Foster; K. Gross; J. Maley; M. Monn; R. Chandrashekar
IDEXX Laboratories
Westbrook, ME, USA

The SNAP® Feline TripleTM test is an enzyme-linked immunosorbent assay (ELISA) for the simultaneous detection of antibodies to Feline Immunodeficiency Virus (FIV), Feline Leukemia Virus (FeLV) antigen and heartworm (HTWM) antigen in feline serum, plasma or whole blood. The FIV antibody assay utilizes a peptide derived from the envelope protein and a recombinant p24 protein. The FeLV and HTWM assays utilize antibodies to detect p27 FeLV antigen and HTWM antigen, respectively.

The purpose of this study was to evaluate the performance of the SNAP Feline Triple test. A total of 462 samples were tested in this evaluation. Various confirmatory tests were performed to assess the true infection status of the samples for each analyte. These include PetChek® FIV for FIV antibody, PetChek® FeLV for FeLV antigen and PetChek® HTWM PF and/or necropsy for feline heartworm. Results showing the performance of SNAP Feline Triple with these samples are summarized in the table below.

Click on the table to see a larger view.


 

For the population of samples tested in this study, the SNAP Feline Triple test had 99.2% sensitivity and 100% specificity for the detection of antibodies to FIV compared to the microtiter plate assay (PetChekFIV). The sensitivity and specificity of the SNAP Feline Triple test for FeLV antigen was 100% and 98.6%, respectively, compared to PetChek FeLV. The sensitivity and specificity of the SNAP Feline Triple test for HTWM antigen was 86.7% and 100%, respectively, compared to PetChek HTWM PF and/or necropsy.

Thus, the SNAP Feline Triple test can be used as a rapid, in-clinic test for the simultaneous detection of antibodies to FIV and both FeLV antigen and heartworm antigen in feline samples.

Speaker Information
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Celine Mainville


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