Effect of Prednisone Administration on Thrombelastography Parameters in Healthy Beagles
Long-term corticosteroid therapy has been associated with an increased risk of thromboembolic complications in dogs. The purpose of this prospective study was to use thrombelastography (TEG) to detect the development of a hypercoagulable state in beagles receiving oral prednisone. We hypothesized that the administration of corticosteroids to healthy dogs would result in a hypercoagulable profile on TEG tracings. To our knowledge, the effect of corticosteroid therapy on TEG tracings has not been previously investigated in dogs.
Six healthy adult beagles were included in the trial. Dogs received 1 mg/kg of prednisone once daily for two weeks, followed by a 6-week washout period and then 4 mg/kg of prednisone once daily for two weeks. TEG tracings were obtained before prednisone administration (baseline), at the end of the washout period and at the end of both corticosteroid trials. TEG analyses were performed in duplicate on citrated whole blood 30 minutes after collection using recombinant human tissue factor at a final concentration of 1:3600. The reaction time (R), the alpha angle (α), the kinetic time (K) and the maximum amplitude (MA) were recorded.
A repeated-measures linear model was used to test for the effect of treatment. Significant results were obtained for K (P=0.0002), α (P=0.0013) and MA (P<0.0001), with tracings compatible with a hypercoagulable profile after corticosteroid trials when compared to baseline. A significant difference between corticosteroid dosages was only detected for MA (P=0.0037).
Further studies are needed to determine the underlying mechanisms of this hypercoagulability.