Abstract

Quinaldine and clove oil are favorite anesthetic agents that have not been study in Midas cichlids, Amphilophus citrinellus. The objective of this study was to determine the effective concentration acute toxicity and comparative efficacy between quinaldine and clove oil in Amphilophus citrinellus. The parameters evaluated were induction and recovery time, haematological and blood biochemical profiles and histopathological study of gill, liver and kidney. The three month old female fish were anesthetized by quinaldine or clove oil in various doses (10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140 and 150 ppm) until reach stage three plane two. The animals were recovered and all samples were collected at 24 hours after induction of anesthesia. The recommend doses for quinaldine is 20 to 150 ppm and for clove oil between 50 and 150 ppm. Clove oil is suggested for handling Amphilophus citrinellus because it has a greater anesthetic effect than quinaldine and provide smoother anesthesia.^1,2,3 However, quinaldine and clove oil should be used carefully because of potential toxicity to the gills and kidneys.

Acknowledgements

This work was financially supported by a grant from Mahidol University, Nakornpathom., Thailand.

References

1.  Brown LA. 1993. Anesthesia and Restraint. In: Fish Medicine, M.K. Stoskopf and W.B Saunders (ed.) 79-90.

2.  Cooke SJ, Suski CD, Ostrand KG, Tufts BL, Wahl DH. 2003. Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides). Aquaculture. 239: 509-529.

3.  Velisek J, Svobodova Z, Piackova V. 2005. Effects of Clove Oil Anaesthesia on Rainbow Trout (Oncorhynchus mykiss). 74: 139-146.