Vesicoureteral reflux (VUR) is the retrograde flow of urine from the bladder to the upper urinary tract which is a common finding in human and animals. In this regard, it is notable that VUR is the focus of more than 150 manuscripts in the literature each year. Twelve rabbits randomly procured with the purpose of inducing and comparing different surgical methods of vesicoureteral reflux. All rabbits were divided into four equal groups of three rabbits in each group. All rabbits were preoperatively evaluated with retrograde cystography for the presence or absence of congenital or acquired vesicoureteral reflux. Rabbits of group 1(control) without any manipulation were kept for comparing with other three experimental groups. Rabbits of group 2, 3 and 4 undergone open surgeries with unroofing the ureter with surgical blade incision of 3-5mm in length without suturing the incised ureter in group 2, sutured with nylon material 5/0 in group 3 and with electrosurgical cutting without suture in group 4 respectively. Postoperatively on days 15th and 30th with cystography evaluated the presence of VUR in all groups. With the result all the animals of group 1, 2 and 4 didn't show any reflux in double contrast cystography but in group 3 reflux in the form of bilateral and unilateral were observed clearly.