The Change in Apoptotic Factor of Synovial Fluid Obtained from Experimentally Induced Osteoarthritis Dogs
World Small Animal Veterinary Association World Congress Proceedings, 2007
Jung-Hyang Sur; Du-Na Hwang; Chi-Ho Yu; Ji-Young Yhee; Geun-Seog Song; Sang-Jin Lee; Jung-Hyang Sur
Konkuk University
South Korea


Osteoarthritis (OA) is the most common form of degenerative joint diseases and a major cause of disability and impaired quality of life in the elderly.


This present study investigated the apoptotic factor in synovial fluid obtained from an experimental osteoarthritic (OA) model and examined the major signaling pathways involved in OA cartilage degradation.


To explore the underlying mechanism, mature beagle dogs underwent a unilateral surgical transection of the cranial cruciate ligament and a medial collateral ligament and medial meniscectomy. The synovial fluid was collected and assay for western blot, lactate dehydrogenase and reactive oxygen species (ROS) were conducted.


Western blot analysis showed that the synovial fluid obtained from osteoarthritis dog showed markedly increased activation of caspase-8 as compared to that of normal. In addition, synovial fluid from osteoarthritis also significantly increased ubiquitin and TRAIL protein expression. Also, osteoarthritis synovial fluid showed high levels of reactive oxygen species (ROS) and increased lactate dehydrogenase (LDH) activity.


Our results suggest that apoptosis may play a pivotal role in osteoarthritis (OA), this apoptotic factor may have a crucial role in cartilage generation and destruction in joint related disease including osteoarthritis. Thus it will be useful for examining the OA pathogens and biomarker to diagnosis.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Jae-won Seol
Chonbuk National University
Jeonbuk, South Korea

MAIN : Abstracts - Poster : Apoptotic Factor of Synovial Fluid
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.


777 W. Covell Blvd., Davis, CA 95616


  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357