In vitro effects of COX-2 Selective Drugs on Oxidative Burst of Blood Neutrophils and Synovial Cells of Dogs by Flow Cytometry
World Small Animal Veterinary Association World Congress Proceedings, 2007
André L. Selmi, DVM, MS, PhD; Cristina Massoco, DVM, MS, PhD; Ana C. Teixeira

Traditional studies performed in dogs using NSAIDs evaluate eicosanoids levels, cytokines and other factors associated to acute inflammation response. The present study is focused on the oxidative burst, which measures the production of reactive oxygen species of blood and synovial leukocytes of dogs. Therefore, the aim of this study was to compare the effect of different NSAIDs and a steroidal drug on the phorbol 12-myristate 13-acetate (PMA)-induced oxidative burst of blood neutrophils and synovial mononuclear cells by flow cytometry technique. The synovial fluid from both stifles and blood from 6 healthy dogs were collected in heparinized tubes and 106 cells were incubated with 2'7'diacetate-diclorofluorescein (DCFH-DA) and carprofen (100 µM), or ketoprofen (100 µM), or hydrocortisone (100 µM), or meloxicam (100 µM) or buffer phosphate solution (negative control) for 30 minutes at 37°C. The comparative analysis of oxidative burst after PMA stimulus showed that carprofen (720 ± 30; p< 0,001), ketoprofen (700 ± 20; p< 0,001), hydrocortisone (716 ± 50 p< 0,01) and meloxicam (1150 ± 20; p< 0,05) were efficacious in attenuating the production of free radical by blood neutrophils when compared to negative control (1562 ± 45). The synovial mononuclear cells showed the same behavior: carprofen (103 ± 8; p< 0,001), ketoprofen (173 ± 9; p< 0,001), meloxicam (268 ± 3 p<0,05) and negative control (290 ± 4). All NSAIDs used in this study showed some inhibitory effect on PMA-induced oxidative burst compared with hydrocortisone, however meloxicam would have a lower trend potency on the oxidative burst process.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

André L. Selmi, DVM, MS, PhD
Universidade Anhembi Morumbi
Sao Paulo, Brazil


MAIN : Abstracts - Poster : Cox-2 Selective Drugs
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.

CONTACT US

777 W. Covell Blvd., Davis, CA 95616

vingram@vin.com

PHONE

  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357
SAID=27