Systemically Applied Allogenic Mesenchymal Stem Cells in Treatment of Radial Segmental Defects
V. Ledecky; M. Ushmirsky; M. Hluchy; K. Ledecka; D. Franka
Department of Surgery, Orthopedics and Roentgenology, University of Veterinary Medicine, Slovak Republic
Allogenic adult mesenchymal stem cells (MSCs) can be used in healing of bone defects. Local application of MSCs showed very strong osteoproductive properties; however other modes, particularly systemic I.V. approach, have not been yet investigated.
To test the hypothesis that systemic application of MSCs can be applied in treatment of radial segmental defect.
Rabbit MSCs were isolated from femurs of adult animal and ex vivo expended. 15 radial defects of 20 mm size were created in 3 groups of experimental rabbits. Grafts containing Tri-Calcium Phosphate (TCP), TCP and MSCs added locally to defect and TCP and MSCs applied systemically I.V. were performed and observed for 12 weeks.
Radiological, histological and histomorphometric evaluation of bone repair was made in present study.
TCP with MSCs applied locally group demonstrated the best radiographic, histological and histomorphometric score of bone healing. TCP and MSCs applied systemically group was evaluated with lower osteoproductive potential. Group grafted with pure TCP revealed only mild osteoproductive properties and the lowest score.
Systemic application demonstrated the ability of MSCs to reach the peripheral bone defect and to amplify the defect healing. Future studies are required to determine the safety and efficacy of MSCs and their precise fate in systemic application.