Laboratorio de Farmacología Vascular de la Sección de Postgrado e Investigación del IPN, Hospital Veterinario El Arca de Pet
Introduction: In dog and cat myocardial infarction results from myocardial ischemia and is a serious problem for, the ischemic patient, because it compromises the ability of the heart to pump blood through loss of contractile mass. Over the past 30 years there has been a concerted effort to identify the mechanism of this pathology, which would make the heart more resistant to this damage.
Objectives: Determine the pathology of the myocardial infarction
Methods: Adult, male wistar rats (250 to 300g) were anesthetized with ketamine HCL (60mg/kg) and acepromazine (1mg/kg IP). Trachea was cannulated, and ventilation was maintained with 100% oxygen by a rodent ventilator, adjust to maintain exhaled CO2 between 3.5 and 5%. A left thoracotomy was performed and suture was placed 3 to 4 mm from the origin of the left coronary artery. Ischemia (30 minutes) was initiated by tightening of the suture around the coronary artery and was followed by 4 hours of reperfusion. Sham-operated rats were prepared identically, except that the suture was not tightened, infarct size (percentage of area at risk) was determine by triphenyltetrazolium chloride staining, the area of infarction do not dye, the statistical method that we can use is with 1-way ANOVAs.
Results: In our lab in this moment we are working with hormones, glycoprotein and desinflamatory drugs.
This is a model easy to use
The results are obtained quickly
The equipment that you use are not expensive
This model offers the opportunity to study the pathology of ischemia and reperfusion