Concerning the importance of eyelid in eye health, deferent methods have been proposed for reconstruction of lid's defects in human and animals. In this study preputial graft has been used for the first time to reconstruct the experimental full- thickness defect of lower eyelid in dog.

This study was carried out on 8 male, mixed-breed healthy dogs, randomly assigned to four equal groups. Following surgical preparation and anesthesia, a 1×1×1 cm triangle was dissected free from the cranial margin of prepuce. A defect with the same size was then created at the margin of lower eyelid, and the graft was sutured in two layers (conjunctiva and skin) to the defect. Histopathological samples were taken on days 7, 8, 10 and 12 postoperatively to evaluate healing stages.

According to the histopathological exam, all the grafts were taken superficially and deeply.

Concerning the results of this study, preputial graft for reconstruction of lower eyelid in full- thickness defects in dog is successful and has favorable functional and cosmetic results.