Angiogenin Enhanced Splenosis Following Splenectomy in Dogs
WSAVA 2002 Congress
*Beatriz Loscertales Martín de Agar, Zhong Qian, Jaime Rodriguez, Kevin McCarthy, William D. Scheer, Rogelio Moncada
*Centro de Cirugía de Mínima Invasión, Avda. de la Universidad, s/n (Campus Universitario)
Cáceres, ES


Because of immune system deficiencies and associated overwhelming postsplenectomy infection, splenic preservation at time of splenectomy by splenorrhaphy, partial resection or autotransplantation is attempted. Splenosis has been shown to maintain some immune and reticuloendothelial functions, but the extent of short and long term beneficial effects of splenosis is undetermined. This canine study uses Angiogenin to enhance volume and function of splenosis.


Nine dogs undergoing splenectomy were divided into three groups, with 3 dogs each, based on the type of autotransplantation of the splenic tissue. In Group 1, an average of 3.5 mg of splenic fragments (2 mm3) were implanted in the peritoneal cavity and abdominal wall. In Group 2, the splenic fragments were immersed in 1ml saline containing 5µm of angiogenin for 15 min before implanted to the peritoneal cavity and abdominal wall. Besides same as in Group 2, an intramuscular injection of 0.5µm angiogenin was given weekly to the animals in Group 3 for 12 weeks. To evaluate for splenosis, 6 months after surgery scintigraphic abdominal and chest scanning was performed using intravenous injection of 1mCi of 99mTC sulfur colloid in all dogs. Blood samples were obtained to determine the presence of Howell-Jolly bodies. Seventy-two hours after the scanning, a laparotomy was performed to harvest all residual splenic tissues in all animals. The splenic tissues were weighed and examined macroscopically and microscopically.


All animals were survived, but one in Group 2 died of unknown cause. Scintigraphy showed either negative (n=2) or slightly positive (n=1) in Group 1; moderately positive (n=2) in Group 2; and either strongly positive (n=2) or moderately positive (n=1) in Group 3. Relative scintigraphic activity is being calculated to compare residual splenic uptake quantitatively among the groups of dogs. Howell-Jolly bodies were detected in all groups, but less prominent in Group 3. Most of the splenic pulps were, in kidney shaped, found in the omentum. The average of harvested splenic pulps was 4.63 gm with Group 1, 5.34 gm with Group, and 7.60 gm with Group 3.


Our preliminary results indicate angiogenin enhances regeneration and growth of autotransplanted splenic tissues following splenectomy. The use of angiogenesis factor may provide a better approach to help protect asplenic and hyposplenic patients from overwhelming postsplenectomy infections.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Beatriz Loscertales Martín de Agar
Centro de Cirugía de Mínima Invasión
Avda. de la Universidad, s/n (Campus Universitario)
Cáceres, Cáceres 10071 ES

Jaime Rodriguez
Louisiana State University Health Science Center

Kevin McCarthy
Louisiana State University Health Science Center

Rogelio Moncada
Louisiana State University Health Science Center

William D. Scheer
Louisiana State University Health Science Center

Zhong Qian
Louisiana State University Health Science Center

MAIN : : Angiogenin Enhanced Splenosis
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.


777 W. Covell Blvd., Davis, CA 95616


  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357