Immunization of Puppies with a New Canine Parvovirus Vaccine using the Variant CPV-2b: Comparison with Three Conventional Vaccines
WSAVA 2002 Congress
*Laure Gardey, Marta Leiva Repiso, Rosa Gil Brun, Virginie Martin, Carles Morales Moliner
*Virbac SA
Carros cedex, Alpes Maritimes, FR


A new modified live vaccine containing a Canine Parvovirus variant CPV-2b was developed in order to better match the current field strains which have replaced the original CPV-2 type and to allow an earlier and efficient immunization in young pups, despite the obstacle of maternally derived antibodies (MDA). The objective of the study was to compare the immunogenicity of four monovalent modified live vaccines, one vaccine containing the CPV-2b variant (vaccine A) and the three other being conventional CPV-2 vaccines, currently marketed in Europe (vaccines B, C and D).


181 puppies aged 5 weeks completed the study in the area of Barcelona. All pups of a same litter were randomly assigned to a vaccine group. Dogs were vaccinated at 5 and 7 weeks (days 0 and 14). Antibody titers to CPV-2, CPV-2a and CPV-2b were determined on D0, D14 and D28 by the hemagglutination inhibition test. At D0, pups were seropositive (SP) when MDA titer were > or = 1:20 and seronegative (SN) when <1:20. Seroconversion (SC) at D14 and D28 was defined as a four-fold increase or greater in titers when SP at D0, or when titer increased from a SN to a SP value. Geometric means CPV-2, 2a and 2b titers were determined and ANOVA was used to compare the mean titers, with the group, time and MDA status as factors. The SC rates were compared using the Chi-square test. Fischer's exact test was used for 2X2 comparisons. Difference was significant at P<0.05.


96% of pups in group A seroconverted regarding CPV-2, 2a and 2b by 7 weeks (D14), when the SC rates with vaccines B, C and D ranged from 49 to 68%. In every case, the SC rate in group A was significantly higher than the one in the other groups. At D28, SC rates in group A regarding CPV-2, 2a and 2b were 92, 96 and 98% when the SC rates in groups B, C and D increased but remained lower than the rates in group A (from 60 to 73%). CPV-2 titers (D14 - D28) were: A (1:4817 - 1:1856), B (1:774 - 1:532), C (1:266 - 1:411), D (1:291 - 1:664). CPV-2a titers were: A (1:6211 - 1:2477), B (1:201 - 1:72), C (1:65 - 1:78), D (1:74 - 1:147). CPV-2b titers were: A (1:6301 - 1:2662), B (1:197 - 1:71), C (1:87 - 1:101), D (1:75 - 1:172). CPV-2a and 2b titers with vaccine A were significantly different when compared to the 3 other vaccines at D14. CPV-2 titers with vaccine A were significantly different when compared to the 3 other vaccines at D14 in pups presenting with MDA.


The presence of MDA at D0 didn't prevent the development of a strong immune response as early as D14 in pups that received the CPV-2b variant vaccine (A), contrary to vaccines B, C and D. The results indicate that an early and high humoral response was achieved with the new CPV-2b vaccine and suggest that a high level of immunization can be expected in pups as early as 5 weeks of age against the current field CPV strains.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

Carles Morales Moliner
Hospital ClĂ­nic Veterinari. Facultat de VeterinĂ ria
Campus UAB. Edifici V
Bellaterra, Barcelona 08193 ES

Laure Gardey
Virbac SA
BP 27
Carros cedex, Alpes Maritimes 06511 FR

Marta Leiva Repiso
Hospital Clinic Veterinari, Universitat Autonoma de Barcelona

Rosa Gil Brun
Laboratorios Virbac SA

Virginie Martin
Virbac SA

MAIN : : Parvovirus Vaccine using the Variant CPV-2b
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.


777 W. Covell Blvd., Davis, CA 95616


  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357