Value of Two Rapid Immunomigration Tests for the Detection of Canine Ehrlichiosis and Heartworm Disease
WSAVA 2002 Congress
*Davoust Bernard, Boni Michaël, Parzy Daniel
*Direction du Service de Santé en région Sud-Est
Lyon, FR


The aim of this study is to evaluate the performances of two rapid immunomigration tests under field conditions. The two diagnostic tests are used for the detection of canine ehrlichiosis caused by Ehrlichia canis and heartworm disease caused by Dirofilaria immitis. The dog population selected for this trial was from Sudan, Africa. This study deals also with some epidemiological data.


In 2000, samples were collected from 38 local dogs from the city of Barbar el Fugarra from the eastern part of Sudan (on the border of Ethiopia).

For ehrlichiosis diagnostic, sera were tested with a Rapid ImmunoMigration (RIM®) test (WITNESS® Ehrlichia) and an ImmunoFluorescent Assay (IFA) as reference technique.

For heartworm disease diagnostic, sera were tested with a Rapid ImmunoMigration (RIM®) test (WITNESS® Dirofilaria) and an Enzyme Linked ImmunoSorbent Assay (ELISA) as reference technique (DiroCHEK®).


The canine ehrlichiosis seroprevalence is 100% (38 dogs positive in IFA - titers  1/40). Correlation with rapid immunomigration test is very strong (r=1, minimum guarantied rm=0.90 with =5%). Only one serum with a IFA titer of 1/40 over the 38 sera tested remains negative with Rapid ImmunoMigration (RIM®) test. Therefore sensitivity is 97.3% and specificity is 100%.

The heartworm disease seroprevalence is 5.4 % (2/37 dogs positive in ELISA). Correlation with Rapid ImmunoMigration (RIM®) test is very strong (r=1, minimum guarantied rm=0.90 with =5%).The sensitivity and the specificity of this rapid test are 100%.


Rapid ImmunoMigration (RIM®) tests present very high performances. They are accurate, easy-to-use and rapid (results in 10 minutes). They can be stored at room temperature and can be easily used under field conditions. They are useful for the diagnostic of asymptomatic heartworm disease and canine ehrlichiosis as well as for the confirmation of a clinical diagnostic. For the first time this study also shows a high prevalence of canine ehrlichiosis and a low prevalence of heartworm disease occurring in Sudan.

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

BONI Michaël
Direction interarmées du Service de Santé des Forces Françaises stationnées à Djibouti

Direction du Service de Santé en région Sud-Est
1 bis Place Bellevue
Lyon, Rhône-Alpes 69998 FR

Daniel PARZY
Unité de Parasitologie de l'Institut de Médecine Tropicale du Service de Santé des Armées - Marseille (France)

MAIN : Hematology/Immunology : Rapid Immunomigration Tests
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.


777 W. Covell Blvd., Davis, CA 95616


  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357