Morphologic and Cytochemical Staining and Ultrastructural Characteristics of Rhinoceros (Cyclura conutus) and Green Iguana (Iguana iguana) Leukocytes
IAAAM 2000
Gabriela Fuentes, DVM; Gerald E. Byrne, Jr., MD; Carolyn Cray, PhD; Julia Zaias, DVM; Kendra Clark, CVT; Susan Decker, BS; Norman Altman, VMD
University of Miami, School of Medicine, Papanicolaou Cancer Research Building, Miami, FL, USA

Abstract

Peripheral blood smears from four adult and one juvenile rhinoceros iguana (Cyclura conutus) and four adult green iguanas (Iguana iguana) were examined after staining with periodic acid-shift (PAS), toludine blue (TB), peroxidase benzadine dihydrochoride (PBD), Giemsa, and Wright-Giemsa. Heterophils, monocytes, and azurophils stained positive for PAS. Monocytes resembled those of other reptilians. Basophils stained positive with toludine blue and were negative for PBD and PAS. Heterophils and azurophils stained positive for PBD. Lymphocytes and eosinophils were seen only in Wright-Giemsa and Giemsa. There was consistency in the cytochemical staining of both species. Transmission electron microscopy revealed matrix within all granulocyte granules, including those of basophils. There are insufficient data in reptilian hematologic parameters and cytochemical characterization. These results clarify improper nomenclature previously reported and provides a reference for future hematologic studies in this species.

References

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Speaker Information
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Gabriela Fuentes, DVM


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