Epithelialization and contraction are two significant parts of natural healing. Cohen isolated a polypeptide from the secretions of the submaxillary salivary glands of adult male mice, which stimulated the proliferation of various epidermal and epithelial tissue both in vivo and in vitro. The goal of this study was to evaluate the healing effects of bovine saliva (as an available saliva) on induced open wounds in dogs. Five dogs (5-10 kg) were used and a 1-2 cm skin defect were created on both sides of the lumbar area. One of two treatments A) bovine saliva and vaseline gel, or B) normal saline and vaseline gel were used on the wounds. Twice a week photographs were taken from the same distance until 30th days post operative. The epithelialization, contraction and healing area were measured and analyzed by SPSS software. The (t) test was used to statistically evaluate the relative speed of healing between treatments, and a linear regression equation was calculated to assess the changes in wound area with time, according to the treatments applied. The (t) test analysis showed there was no significant difference between the treatments.