Effect of Vitamin C and E on Renal Injury in Pig Autotransplantation Model
World Small Animal Veterinary Association World Congress Proceedings, 2006
C.S. Park1, M.J. Kim2, M.H. Jun2, J.Y. Lee2, S.W. Cho2, S.M. Jeong2, M.C. Kim2
1Div. of Animal Sci. & RCTCP, Chungnam Natl. Univ., South Korea; 2Coll. of Vet. Med., Chungnam Natl. Univ.

Introduction

Ischemia-reperfusion (I/R) injury is a major cause of renal failure and renal graft rejection (1,2). The purpose of this study is to determine that antioxidants vitamin C and E therapy provides protection against I/R injury in pig kidney during kidney transplantation.

Materials and methods

In control group (n=3), after the left kidney was removed, all arteries are perfused using hepa-saline (heparine 1000 IU + saline 500 ml) followed by autotransplantation. And then right nephrectomy performed. In treatment group (n=3), vitamin C (1,000 mg/head/day, IM) and vitamin E (100 IU/head/day, PO) combination were given for 2 days before operating. After the left kidney was removed, all arteries are perfused using hepa-saline including vitamin C 1,000 mg. After implantation of the renal autograft, right kidney was removed.

Results

The level of BUN increased to 81.0±5.57 mg/dl at day 1 the control group, and then increased gradually and was not normalized until 7 days after renal autotransplantation. While in the treatment group, the level of BUN went up to 53.50±16.23 mg/dl at day 1 and then slightly decreased. In the control group, serum creatinine levels increased to 6.80±2.22 mg/dl after 1 day of renal autotransplantation and then decreased slightly and were not normalized until 7 days of operation. The serum creatinine level in the treatment group increased 4.00±0.28 mg/dl after 1 day of renal autotransplantation and then slightly decreased to 3.05±0.07 mg/dl by 7 days of operation.

Conclusion

It was considered that antioxidants vitamin C and E therapy may have roles on the attenuation of I/R injury and recovery of renal function in the renal transplantation model in growing pigs.

References

1.  Casanova D et al. 2002. Transplant Proc 34: 45-46.

2.  Sarin PK et al. 2003. Transplant Proc 35: 35-36.

Speaker Information
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C.S. Park
Div. of Animal Sci. & RCTCP
Chungnam Natl. Univ.
Yoosung-gu, Daejeon, South Korea


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