Who's Behind the Mask and the Cape? Asian Leopard Cat's Agouti Allele Affects Coat Colour Phenotype in Bengal Cat Breed
Tufts' Canine and Feline Breeding and Genetics Conference, 2013
L.C. Gershony1; A. Cortes1; M.C.T. Penedo2; B.W. Davis3; W.J. Murphy3; L.A. Lyons1
1Department of Population Health and Reproduction and 2Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis, CA, USA; 3Department of Veterinary Integrative Biosciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA

Coat colours and patterns are highly variable in cats and determined by several genes. The charcoal coat pattern inheritance in Bengal cats appears as an incomplete melanism, thus the agouti signalling protein gene (ASIP) was investigated as a candidate gene for this phenotype. DNA was isolated from buccal swabs obtained from 72 Bengal cats, where 49 were presumed to be charcoal. The coding region of ASIP was amplified by polymerase chain reaction and subsequently directly sequenced. The resulting sequences were compared to that of ten Asian leopard cats and three control domestic cats. Polymorphisms were investigated within the gene. Two non-synonymous SNPs were observed in exon 2 (c.41G>C and c.142T>C) when comparing the control domestic cat sequence with the leopard cat sequence, resulting in amino acid changes in the leopard cat (Cys14Ser and Ser48Pro, respectively). One synonymous single-nucleotide polymorphism (SNP) was found in exon 3, substituting a cytosine for adenine in the leopard cat (c.162C>A). Forty-three charcoal cats presented as compound heterozygotes at ASIP, consisting of an Asian leopard cat allele and a domestic cat non-agouti allele (a). The compound heterozygote state suggests that the interaction between the Asian leopard cat allele and the domestic cat allele allowed for the recessive non-agouti allele to influence the markings of the hybrid Bengal cat, producing a darker, yet not completely melanistic coat pattern. This study presents the first validation of a Leopard cat allele segregating in the Bengal breed, affecting the overall phenotype of the pelage.

1.  Further investigation should be conducted to assess similar interactions in other genes, and how they would affect the accuracy of genetic tests within this breed.

2.  Further investigation should be performed to better illuminate the potential allelic interactions, and consequential phenotypic expression, within this hybrid breed.

  

Speaker Information
(click the speaker's name to view other papers and abstracts submitted by this speaker)

L.C. Gershony
Department of Population Health and Reproduction
School of Veterinary Medicine, University of California
Davis, CA, USA


MAIN : Posters : Agouti Allele & Coat Color
Powered By VIN

Friendly Reminder to Our Colleagues: Use of VIN content is limited to personal reference by VIN members. No portion of any VIN content may be copied or distributed without the expressed written permission of VIN.

Clinicians are reminded that you are ultimately responsible for the care of your patients. Any content that concerns treatment of your cases should be deemed recommendations by colleagues for you to consider in your case management decisions. Dosages should be confirmed prior to dispensing medications unfamiliar to you. To better understand the origins and logic behind these policies, and to discuss them with your colleagues, click here.

Images posted by VIN community members and displayed via VIN should not be considered of diagnostic quality and the ultimate interpretation of the images lies with the attending clinician. Suggestions, discussions and interpretation related to posted images are only that -- suggestions and recommendations which may be based upon less than diagnostic quality information.

CONTACT US

777 W. Covell Blvd., Davis, CA 95616

vingram@vin.com

PHONE

  • Toll Free: 800-700-4636
  • From UK: 01-45-222-6154
  • From anywhere: (1)-530-756-4881
  • From Australia: 02-6145-2357
SAID=27