Unraveling the Sources of Genetic Structure Within Breeds
Tufts' Canine and Feline Breeding and Genetics Conference, 2013
Pam Wiener, PhD
The Roslin Institute, The Royal (Dick) School of Veterinary Studies, Division of Genetics and Genomics, University of Edinburgh, Scotland, UK

Genetic analyses of domesticated animal species have proved very useful for determining relationships between breeds (Wiener et al. 2004), for illuminating the processes underlying the domestication process (Wiener, Wilkinson 2011), and for identifying genes associated with specific traits (Georges 2007). An important tool is the use of clustering-based population genetic methods, in which populations are determined based on the genetic makeup of individuals, without prior population labelling. These techniques have been applied to domesticated animal species in a number of studies and in most cases have demonstrated good correspondence between breeds and genetically defined populations. Use of this approach has proven to be particularly useful for identifying animals that do not fit the general genetic profile of a given breed - for example, crossbred or misclassified individuals.

Within-Breed Genetic Differentiation

In some cases, however, clustering techniques have revealed population structure below the breed level, such that separate groupings are identified within breeds. This was demonstrated in an analysis of British pig breeds, in which the British Saddleback breed showed internal genetic structure (Wilkinson et al. 2008). There appeared to be greater differentiation between the two British Saddleback clusters than between some breed pairs (Figure 1). A similar finding was found for several British chicken breeds (Wilkinson et al. 2011), in which within-breed differentiation was associated with different morphological types for some breeds and with different flocks in others (Figure 2). The latter pattern indicates restricted gene flow between breeders, which can lead to high rates of inbreeding.

Figure 1. A neighbour-joining tree of British pigs constructed from allele-sharing distances among all individuals
Figure 1. A neighbour-joining tree of British pigs constructed from allele-sharing distances among all individuals

Bootstrap values greater than 500 are shown (out of 1000). British Saddleback individuals are found in two separate clusters. Reproduced from Wilkinson et al. (2008).
 

Figure 2. Individual assignment based on clustering analysis at K = 35
Figure 2. Individual assignment based on clustering analysis at K = 35

Histograms demonstrate the proportion of each individual's genome that originated from each of 24 populations. Each individual is represented by a vertical line corresponding to its membership coefficient (q). Genetic structure is seen within breeds such as Araucana, Leghorn, Maran, Silkie and Sussex. Reproduced from Wilkinson et al. (2011).
 

Several recent studies in dogs have also identified within-breed differentiation, which derives from several sources. Quignon et al. (2007) analysed American and European samples from four breeds and demonstrated a clear genetic separation of US and EU golden retrievers. They also identified genetic differentiation within Bernese mountain dogs, but it was not clearly associated with geographical origin. Two other breeds in that study (Flat-coated retrievers and Rottweilers) did not show evidence of genetic structure. In other cases, genetic differentiation is associated with phenotypic traits. Bjornfeldt et al. (2008) identified strong genetic differentiation in poodles due to size and coat colour. Standard poodles were clearly genetically distinct from all other poodles, while the smaller poodles were differentiated from each other based on a combination of size and coat colour. A study on Schnauzer breeds revealed a similar pattern of differentiation (Streitberger et al. 2011); the authors found that Giant Schnauzers were strongly differentiated from the other Schnauzer breeds, while the smaller Schnauzers clustered based on both coat colour and size. Mellanby et al. (2013) also demonstrated genetic structure within UK Cavalier King Charles spaniels, although the source of the differentiation was not clear. Preliminary analysis of UK Labrador retrievers indicates within-breed genetic differentiation related to the role of dogs (i.e., working gun dogs versus pets) as well as phenotypic characteristics (unpublished results).

Implications for Managing Recessive Diseases

Strong population structure may lead to high levels of inbreeding by creating partially independent subpopulations with relatively small effective population sizes, increasing the role of genetic drift. This can thereby increase the overall levels of homozygosity, and thus may also increase the numbers of individuals homozygous for recessive disease alleles. Management practices that increase mixing within the breed will reduce overall levels of inbreeding and therefore may help reduce the levels of such diseases. Somewhat ironically, in rare breeds, management strategies that involve reduced breeding from a segment of the breed that carries known disease-associated variants may exacerbate the problem at other loci by reducing the effective population size (Collins et al. 2011), and thus these strategies must be designed with care and forethought.

Implications for Genetic Association Studies and Genetic Evaluation

It is well established that the existence of genetic structure can lead to spurious associations in genome-wide association studies if the trait of interest is not evenly distributed with respect to genetic subgroups (Lander, Schork 1994; Price et al. 2006). Therefore, it is recommended that in such cases, stratification should be accounted for (Price et al. 2010). Population structure may also influence the implementation of genomic evaluation schemes, in which breeding decisions are based on genomic marker information; however, the implications of such structure are less clear in this case. For example, Daetwyler et al. (2012) conclude that the accuracy of prediction may be reduced by accounting for population stratification in some situations (e.g., low- or medium-density markers). Further study is required on this issue.

References

1.  Björnfeldt S, Hailer F, Nord M, Vilà C. Assortative mating and fragmentation within dog breeds. BMC Evolutionary Biology. 2008;8:28.

2.  Collins LM, Asher L, Summers J, McGreevy P. Getting priorities straight: risk assessment and decision-making in the improvement of inherited disorders in pedigree dogs. The Veterinary Journal. 2010;189:147–154.

3.  Daetwyler HD, Kemper KE, van der Werf JJJ, Hayes BJ. Components of the accuracy of genomic prediction in a multi-breed sheep population. Journal of Animal Science. 2012;90:3375–3384.

4.  Georges M. Mapping, fine mapping, and molecular dissection of quantitative trait loci in domestic animals. Annual Review of Genomics and Human Genetics. 2007;8:131–162.

5.  Lander ES, Schork NJ. Genetic dissection of complex traits. Science. 1994;265:2037–2048.

6.  Mellanby RJ, Ogden R, Clements DN, French AT, Gow AG, et al. Population structure and genetic heterogeneity in popular dog breeds in the UK. The Veterinary Journal. 2013;196:92–97.

7.  Quignon P, Herbin L, Cadieu E, Kirkness EF, Hédan B, et al. Canine population structure: assessment and impact of intra-breed stratification on SNP-based association studies. PLoS One. 2007;12:e1324.

8.  Price AL, Patterson NJ, Plenge RM, Weinblatt ME, Shadick NA, et al. Principal components analysis corrects for stratification in genome-wide association studies. Nature Genetics. 2006;38:904–909.

9.  Price AL, Zaitlen NA, Reich D, Patterson NJ. New approaches to population stratification in genome-wide association studies. Nature Reviews Genetics. 2010;11:459–463.

10. Streitberger K, Schweizer M, Kropatsch R, Dekomien G, Distl O, et al. Rapid genetic diversification within dog breeds as evidenced by a case study on schnauzers. Animal Genetics. 2011;43:577–586.

11. Wiener P, Burton D, Williams JL. Breed relationships and definition of British cattle: a genetic analysis. Heredity. 2004;93:597–602.

12. Wiener P, Wilkinson S. Deciphering the genetic basis of animal domestication. Proceedings of the Royal Society B. 2011;278:3161–3170.

13. Wilkinson S, Haley CS, Alderson L, Wiener P. An empirical assessment of individual-based population genetic statistical techniques: application to British pig breeds. Heredity. 2008;106:261–269.

14. Wilkinson S, Wiener P, Teverson D, Haley CS, Hocking PM. Characterization of the genetic diversity, structure and admixture of British chicken breeds. Animal Genetics. 2011;43:552–563.

  

Speaker Information
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Pam Wiener, PhD
The Roslin Institute
The Royal (Dick) School of Veterinary Studies
Division of Genetics and Genomics
University of Edinburgh
Scotland, UK


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